This study investigated the influence of the production system (conventional vs. organic), the grass consumption level and the ageing period (7 and 14 days) on beef quality. Three groups of samples from Limousin × Avileña calves were analysed: F100, formed by animals fed 100% on forage; F74, formed by animals fed on an average amount of forage of 74%; and F35, formed by animals fed on straw fodder (35%) and concentrate (65%). The results showed that the higher the grass content, the lower the fat oxidation and the higher the n-3 content, but also the higher the SFA level, the initial Warner-Bratzler shear force (WBSF), and the more residue it leaves on chewing. As for the effect of production system, organic samples showed higher intramuscular fat content and lower moisture and MUFA content. These samples were darker and showed lower values for flavour quality. Among the organic samples, F100 had higher n-3 and CLA content and higher values for colour, hardness, odour and flavour quality. Increased ageing time may improve the sensory characteristics of the meat, especially in the case of the F100 samples. The results pointed out that F100 samples aged at least 14 days showed the best physico-chemical, nutritional and sensory characteristics.

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Insect larval meal is an increasingly common protein source in poultry systems. In this study, the effect of replacing soybean meal with Tenebrio molitor larval meal on the performance of slow-growing chickens was assessed. A total of 128 one-day-old chickens (Colorield) were randomly divided into a control group (C) (n = 64), fed with soybean meal, and an experimental group (TM) (n = 64), fed with T. molitor larvae meal. The chicks were slaughtered after 95 days. Three different isoenergetic and isoproteic diets (F1, F2 and F3) were used for each group. The F1 diet resulted in higher body weight gain and higher feed and water intakes in group C, but a lower feed conversion ratio. Contrarily, diets F2 and F3 did not produce differences in the studied parameters between the two groups, except for body weight gain in the case of diet F2, which was highest in group C. Therefore, weight gain and feed and water intakes were significantly higher in group C, but there were no differences in feed conversion ratio or live weight. In conclusion, the total replacement of soybean meal with T. molitor larvae meal resulted in a reduction in feed intake and a consequent reduction in weight. During this period, partial rather than total substitution may be recommended.

Nowadays, there is a growing demand for high-quality vegetal protein food products, such as pulses and lentils in particular. However, there is no scientific evidence on the nutritional and morphometric characterization of the main lentil cultivars in the Western Mediterranean area. For this reason, the aim of this work is to carry out a morphometric and nutritional characterization of the main Spanish lentil cultivars. Nutrient content assessment was performed on dry matter. The results showed that all studied cultivars are large and heavy lentils, except for the cultivar “Pardina”. They have high protein levels, ranging from 21% to 25%, which is higher than those found in any other pulse, as well as a high carbohydrate content, greater than 59% in all cases. Fiber content was higher than expected in “Armuña” and “Rubia Castellana” cultivars, ranging from 6% to 6.6%, and exceptionally high in the case of the cultivar “Pardina”, which reached 7.8%. Conversely, very low values were found for fat content, varying between 0.5% and 0.9%. Ca, Fe and Mg levels were remarkably higher (from 550 ppm to 851 ppm, from 98 ppm to 139 ppm and from 790 ppm to 989 ppm, respectively) than those found for other lentil cultivars, especially the high Mg content in the cultivars “Jaspeada” and “Microjaspeada”, both above 955 ppm. Clear differentiation was found between the cultivars “Rubia Castellana”, “Pardina” and those included in the Protected Geographical Indication (PGI) “Lenteja de la Armuña”. Overall, lentil cultivars included in the PGI “Lenteja de la Armuña” showed better morphometric and nutritional characteristics than cultivars “Pardina” or “Rubia Castellana”.

The accurate identification of crops is essential to help environmental sustainability and support agricultural policies. This study presents the use of a Spanish radar mission, PAZ, to classify agricultural areas with a very high spatial resolution. PAZ was recently launched, and it operates at X band, joining the synthetic aperture radar (SAR) constellation along with TerraSAR-X and TanDEM-X satellites. Owing to its novelty and its ability to classify crop areas (both taking individually its time series and blending with the Sentinel-1 series), it has been tested in an agricultural area of the central-western part of Spain during 2020. The random forest algorithm was selected to classify the time series under five alternatives of standalone/fused data. The map accuracy resulting from the PAZ series standalone was acceptable, but it highlighted the need for a denser time-series of data. The overall accuracy provided by eight PAZ images or by eight Sentinel-1 images was below 60%. The fusion of both sets of eight images improved the overall accuracy by more than 10%. In addition, the exploitation of the whole Sentinel-1 series, with many more observations (up to 40 in the same temporal window) improved the results, reaching an overall accuracy around 76%. This overall performance was similar to that obtained by the joint use of all the available images of the two frequency bands (C and X).

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Six cows managed under extensive grazing conditions were used to study the effect of moving the animals to a higher grazing density on the circadian rhythms of temperature (T), heart rate (HR), and activity (ACT), which were recorded by implantable bio-loggers. Cows were maintained at a density of 1.5 livestock units per hectare (LSUs/ha; low density, LD) until they were moved to a grazing area at 128 LSUs/ha (high density, HD). Animals were implanted subcutaneously with a T, HR, and ACT bio-logger, which was programmed to record data at 5-min intervals. For each animal, cosinor rhythmometry (the study of circadian rhythms by fitting a sine wave to a time series) was applied to the data recorded over 5 days in LD and HD. Mean Midline Estimating Statistic of Rhythm (MESOR; the average value around which the variable oscillates), amplitude (difference between the peak and the mean value of a wave), and acrophase (timing of peak activity) were calculated and evaluated statistically. Differences between mean day and nighttime values, and mean LD and HD values were calculated. Cows presented cosinor curves that fit a 24-h rhythm (p < 0.001) in T, HR, and ACT at both densities. MESOR (T: 37.98 vs. 38.02°C; HR: 69.12 vs. 65.91 bpm; ACT: 49.39 vs. 40.41 mg, for LD and HD, respectively) and amplitude (T: 0.28 vs. 0.28°C; HR: 4.12 vs. 3.14 bpm; ACT: 18.14 vs. 11.28 mg, respectively) did not differ significantly between the two densities; however, significant (p < 0.05) differences between densities occurred in the acrophase of the three variables; specifically, the T acrophase was 2 h later at HD (22:45 h) than LD (20:45 h), and HR (LD: 19:51; HD: 16:49 h) and ACT acrophases 3 and 2 h earlier at HD than LD (LD: 14:47; HD: 12:49 h), respectively. T and ACT differed significantly (p < 0.01) between daytime (mean ± SE; 37.92 ± 0.19°C, 40.39 ± 4.74 mg) and nighttime (38.14 ± 0.17°C, 29.93 ± 5.66 mg). In conclusion, our study suggests that a high animal grazing density might exacerbate the social competence for valuable resources for animals, resulting in shifting the circadian rhythmicity of temperature, heart rate, and activity of the cows, advancing or delaying their acrophases.

No abstract