Considerable evidence indicates a critical role for dopamine in the reinforcing effects of cocaine. Because dopamine has been shown to be a critical modulator of gap junction communication in both eye and brain, we sought to examine whether extended intravenous cocaine self-administration would affect the expression of gap junction channel-forming proteins (connexins). Using ELISA, Western analysis, immunohistochemistry, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), and non-radioactive in situ hybridization, we demonstrate that withdrawal from chronic cocaine self-administration causes lasting changes in connexin32 (Cx32) expression in the nucleus accumbens and hippocampus at 2, 7 and 21 days after the last cocaine injection. A sustained decrease in Cx32 protein and mRNA levels is noted in areas that have been implicated in cocaine craving (i.e. nucleus accumbens and subfields of the hippocampal formation). A progressive increase in gap junction protein and mRNA expression is noted in areas that become hyperexcitable after chronic cocaine exposure (i.e. CA1 hippocampal neurons). We speculate that gap junction communication may be critically involved in reinforcement processes and neuroadaptive changes produced by drugs of abuse.
The Tinkertoy test (TTT) has often been used to assess executive function. Despite its clinical importance, there are few published normative data for it. Thus, the primary aim of this study was to fill this gap. Moreover, as there exists a sex difference in many cognitive abilities and neuropsychological tests, a secondary aim was to examine whether sex influences TTT performance. We administered the TTT to 25 healthy men and 25 healthy women whose average age was 28 years. Performances were scored based upon Lezak’s (1982) original TTT criteria. On average, our participants used 43 pieces to complete their construction (SD=8), with a range of 21–50, and their complexity scores ranged from 7 to 12, with a mean score of 9.68 (SD=1.35). Overall performance did not differ based on sex; yet, when examining individual scoring criteria, we found that men scored significantly higher on the symmetry measure. Efforts towards the development of adequate normative data for the TTT and different tests of executive functioning are crucial to neuropsychologists’ and other healthcare providers’ ability to reliably diagnose and treat disorders of cognition that affect executive function. The present data go some way towards enhancing the utility of the TTT.
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