An adrerml.spe¢ifie protein reacting with autoantibodi~ in the s:ra of patients with adult onset Addison's dire.am has I:P..¢n purified from human e.drenal 81ands, The protein, real.we. 35K, hits the biochemical characteristi~ of steroid 21.hydroxylase and reacts on W~tern blast with rabbit antibodies to recombinant 21.hydroxylase, Absorption of the native human 55K adrenal protein with human adrenal autoantibodies prevented the subsequent r~ction of the 55K protein with rabbit :-ntibodies to 21-hydroxylase in W~tcrn blot analysis. In addition, human adrenal autoantibodi~ reacted with recombinant 2l-hydroxylas¢ expressed in y~st, These data inditer that the adrenal specific enzyme steroid 21.hydroxyluse is a major uutoanti~n involved in adult on~t autoimmune Addison's dire.ate,
Resistive particle counting has been developed for the accurate sizing and counting of mitochondria in solution. The normal detection limit with a 30 ~ aperture is 0.48 # diameter, or 0.056 #* particle volume The mean volume of rat liver mitochondria was 0.42/~s or 0.93 # in diameter. The average value for numbers of particles per milligram of mitochondrial protein was 4-.3 X 10 ~, and per gram of rat liver was about 11 X 101°. These values compare satisfactorily with those derived by light microscopy and electron microscopy. The mean volume for mitochondria from rat heart was 0 60 #3 and from rat kidney cortex, 0.23 N~. These values agree within 15 % of those determined by electron microscopy of whole tissue.
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