The efficacy of hydrogen peroxide (H2O2) in controlling fungal infections (saprolegniasis) in the eggs of golden shiner Notemigonus crysoleucas was assessed in two experiments. Golden shiner eggs were exposed in a 15‐min static bath (21°C) to 0, 50, 100, or 200 mg H2O2/L in the first experiment and to 0, 200, 400, and or 800 mg/L in the second experiment. All treatments were based on the amount of active ingredient (30% active ingredient concentration of H2O2) in a single treatment. Three replicates of each concentration were used in each experiment. The objective was to determine the H2O2 concentration that would result in optimum hatching success. The hatching rate significantly increased in each treatment level until 800 mg/L. The mean ± SE percent egg hatchability was 72.3 ± 8.55% and 68.2 ± 5.03% at 400 and 800 mg/L, respectively. Regression analysis revealed the peak treatment level to be between 400 and 800 mg/L.
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