Use of Hydrogen Peroxide to Improve Golden Shiner Egg Hatchability

Abstract

The efficacy of hydrogen peroxide (H2O2) in controlling fungal infections (saprolegniasis) in the eggs of golden shiner Notemigonus crysoleucas was assessed in two experiments. Golden shiner eggs were exposed in a 15‐min static bath (21°C) to 0, 50, 100, or 200 mg H2O2/L in the first experiment and to 0, 200, 400, and or 800 mg/L in the second experiment. All treatments were based on the amount of active ingredient (30% active ingredient concentration of H2O2) in a single treatment. Three replicates of each concentration were used in each experiment. The objective was to determine the H2O2 concentration that would result in optimum hatching success. The hatching rate significantly increased in each treatment level until 800 mg/L. The mean ± SE percent egg hatchability was 72.3 ± 8.55% and 68.2 ± 5.03% at 400 and 800 mg/L, respectively. Regression analysis revealed the peak treatment level to be between 400 and 800 mg/L.

Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner

“…Currently H 2 O 2 is commonly used by salmon farmers at concentrations between 1500 and 2000 mg/L as a bath treatment at ambient water temperatures (Kiemer & Black, 1997). Other uses of H 2 O 2 in aquaculture include the disinfection of fish eggs and microbial control (Bozwell et al, 2009;Matthews et al, 2012). Moreover, despite being a powerful oxidizer, it is rapidly decomposed in water, leaving no toxic products (Arvin & Pedersen, 2015).…”

A Fish of Weedy Waters: Golden Shiner Biology and Culture

“…Typically, eggs are treated daily (until eyed) with a formalin solution to prevent fungus. Alternatively, hydrogen peroxide could be used (Bozwell et al ). Although it has not been tested experimentally on golden shiner eggs, copper sulfate could be another option for fungus control (Straus et al ).…”

“…Typically, eggs are treated daily (until eyed) with a formalin solution to prevent fungus. Alternatively, hydrogen peroxide could be used (Bozwell et al 2009).…”

Effect of Single or Double Exposures to Hydrogen Peroxide or Iodine on Salmonid Egg Survival and Bacterial Growth

“…For example, treatments of 0.5% to 1% for 15-60 min controlled fungal growth in rainbow trout Oncorhynchus mykiss eggs (Schreier et al 1996;Barnes et al 1998). Other experiments indicate that effective treatment levels vary among species (Yamamoto et al 2001), and lower concentrations are needed for species such as channel catfish Ictalurus punctatus (Mitchell et al 2009) or golden shiner Notemigonus crysoleucas (Bozwell et al 2009). Differences in H 2 O 2 sensitivity among rainbow trout strains have also been noted (Gaikowski et al 1998).…”