Background and Aims:New Zealand is exposed to relatively high solar ultraviolet (UV) radiation; such high irradiances of UV radiation having the potential to change the biochemical composition of plants. The aim of this study was to investigate the effects of UV radiation and the role of canopy leaves on berry biochemical composition in Vitis vinifera var. Sauvignon Blanc. Sauvignon Blanc is the major grape variety grown in New Zealand. Methods and Results: Leaves were removed from around the fruiting zones of vines and screens that altered UV radiation exposures were placed over the grape bunches. Samples taken throughout development were analysed for changes in total phenolic compounds (including flavonols), amino acids and methoxypyrazines. Total phenolic compounds increased substantially in response to UV-B exposure and this was reflected in changes taking place within the skins of the berries. Flavonol levels were determined by UV-B radiation exposure and accumulated to maximum concentrations at veraison, subsequently declining to harvest. UV radiation did not have a significant effect on the majority of amino acids or methoxypyrazine concentrations. The most noticeable change in amino acid and methoxypyrazine accumulation was caused by the presence of leaves over the fruiting zone, retaining these leaves maintained significantly higher concentrations in the berries at harvest. Conclusions: UV-B radiation determines the composition of flavonols in the skins of grapes. Amino acid and methoxypyrazine concentrations are not predominantly determined by UV-B, but retention of leaves over the fruiting zone promotes their accumulation in berries. Significance: Canopy manipulations are routinely used commercially in the vineyard to help control vigour and reduce disease pressure. The findings presented here are important for viticulturists to understand how management of the vine leaf canopy can determine the biochemical composition of the grapes and can therefore, ultimately affect wine quality. Gregan et al.Effects of ultraviolet on grape berry biochemistry 227

We examined the influence of short-term exposure of different UV wavebands on the fine-scale kinetics of hypocotyl growth of dim red light-grown cucumbers (Cucumis sativus L.) and other selected dicotyledonous seedlings to evaluate: (1) whether responses induced by UV-B radiation (280-320 nm) are qualitatively different from those induced by UV-A (320-400 nm) radiation, and (2) whether different wavebands within the UV-B elicit different responses. Responses to brief (30 min) irradiations with 3 different UV wavebands all included transient inhibition of elongation during irradiation followed by wavelength specific responses. Irradiations with proportionally greater short wavelength UV-B (37% of UV-B between 280 and 300 nm) induced inhibition of hypocotyl elongation within 20 min of onset of irradiation, while UV-B including only wavelengths longer than 290 nm (and only 8% of UV-B between 290 and 300 nm) induced inhibition of hypocotyl elongation with a lag of 1-2 h. The response to short wavelength UV-B was persistent for at least 24 h, while the response to long wavelength UV-B lasted only 2-3 h. The UV-A treatment induced reductions in elongation rates of approximately 6-9 h following exposure followed by a continued decline in rates for the following 15-18 h. Short wavelength UV-B also induced positive phototropic curvature in both cucumber and Arabidopsis seedlings, and this response was present in nph-1 mutant Arabidopsis seedlings defective in normal blue light phototropism. Reciprocity was not found for the response to short wavelength UV-B. The short wavelength and long wavelength UV-B responses differed in dose-response relationships and both short wavelength responses (phototropic curvature and elongation inhibition) increased sharply at wavelengths below 300 nm. These results indicate that different photosensory processes are involved in mediating growth and morphological responses to short wavelength UV-B (280-300 nm), long wavelength UV-B (essentially 300-320 nm) and UV-A. The existence of two separate types of hypocotyl inhibition responses to UV-B, with one that depends on the intensity of the light source, provides alternate interpretations to findings in other studies of UV-B induced photomorphogenesis and may explain inconsistencies between action spectra for inhibition of stem growth.

The effects of blue light and calcium on elongation of hypocotyl segments of Cucumber (Cucumis sativa L. cv Burpee's Pickier) were studied. Cucumber seedlings grown in dim red light showed a rapid decline in the rate of hypocotyl elongation when irradiated with high intensity (100 micromoles per square meter per second) blue light. In intact, 4-day-old seedlings the inhibition began within 2 minutes after the onset of bluelight irradiation and reached a maximum of approximately 55% within 4 minutes. Hypocotyl segments cut from 4-day-old seedlings also showed an inhibition of elongation in response to blue light when segments were floated on aqueous buffer and exposed to blue light for 3 hours. In the presence of 2 micromolar indole-3-acetic acid, blue light caused a 50% inhibition of elongation. Buffering free calcium in the incubation medium with 0.1 millimolar ethylene glycol bis(-aminoethyl ether)-N,N,N',N'-tetraacetic acid eliminated the blue-light inhibition of segment elongation. Several experiments confirmed a specific requirement for calcium for the blue-light-induced inhibition of segment elongation. Treating segments with 0.2 micromolar fusicoccin abolished the inhibition of elongation by blue light as did buffering the medium at pH 4. Adding 1 milimolar ascorbate to incubation medium also eliminated the inhibition of segment elongation caused by blue light. Several compounds implicated in cellwall redox reactions alter the magnitude of the blue-light-induced inhibition. The activity of peroxidase isolated from the cell-wall free space of cucumber hypocotyls was inhibited by ascorbate and low pH. The results are consistent with the hypothesis that blue light inhibits elongation by inducing an increase in cell-wall peroxidase activity and implicate calcium ions in the response to blue light.It has been known for some time that high-intensity BL3 causes a significant inhibition of stem elongation in stems of dicot seedlings (5,11,18). In dark-grown cucumber seedlings, BL at 4 W m-2 1s-(approximately 15 ,umol m-2 s-') causes inhibition of stem elongation with a lag time of only 20 s (5). A high fluence of BL given over a period of 5 min inhibits elongation by more than 70% (5). After BL irradiation is terminated, the growth rate recovers to the preirradiation control rate within 30 min. The response is not mediated by phytochrome (5, 18), and a similar response has been observed in other species and in deetiolated cucumber (5,11 rigidity and concluded that the stem becomes more rigid when growth is inhibited by BL. It was concluded that this increase in rigidity was caused at least in part by increased turgor pressure in the cells of the hypocotyl. Cosgrove's work (5) also indicated a limitation to studies of stem elongation using hypocotyl segments cut and floated on aqueous medium. The growth rate of cut segments was at most 20% the rate seen for intact plants, and the BL-induced inhibition of elongation was never more than 35%. When auxin was included in the incubation medium (to obtain a highe...

The regulatory pigment phytochrome induces rapid and opposite growth changes in different regions of etiolated maize seedlings: it stimulates the elongation rate of coleoptiles and inhibits that of mesocotyls. As measured by a quantitative immunoassay, phytochrome also promotes rapid and opposite changes in the extractable content of a Mr 98,000 anionic isoperoxidase in the cell walls of these same organs: it induces a decrease of this peroxidase in coleoptiles and an increase in mesocotyls. The peroxidase changes precede the growth changes. As measured by video stereomicroscopy or a position transducer, red light (R), which photoactivates phytochrome, stimulates coleoptile elongation with a lag of about 15-20 min and suppresses mesocotyl growth with a lag of 45-50 min. R also induces a 50% reduction in the extractable level of the anionic peroxidase in coleoptile walls in less than 10 min and a 40% increase in the level of this peroxidase in mesocotyl walls within 30 min. Ascorbic acid, an inhibitor of peroxidase activity, blocks the effects of R on mesocotyl section growth.These results are relevant to hypotheses that postulate that certain wall peroxidases can participate in light-induced changes in growth rate by their effects on wall extensibility.The regulatory pigment phytochrome can initiate rapid growth changes in plants. In etiolated seedlings of oats, maize, and other grasses, the photoactivation of phytochrome by red light (R) stimulates two distinct growth responses: it promotes coleoptile elongation and inhibits mesocotyl elongation (1).Changes in the extensibility of cell walls can help to mediate certain environmentally stimulated growth changes in plants (2), including those induced by phytochrome (3). Cell wall extensibility is at least partially controlled in many plants by one or more wall-localized enzymes that catalyze the formation or breakage of cell wall bonds (4). Consistent with this finding, several authors have noted that there is an inverse correlation between wall peroxidase activity and the growth of cell walls (5); e.g., when cucumber hypocotyl elongation is inhibited by blue light, there is an increase in wall peroxidase activity (6). The inverse correlation is consistent with the function of wall peroxidases in cross-linking wall macromolecules (7). To the extent peroxidases can decrease wall extensibility, they may help to mediate the inhibitory effects Ca2+ can have on wall extensibility (8), for Ca2+ can stimulate both the activity and secretion of wall peroxidases (9).There are numerous wall isoperoxidases, and there has been little or no previously published information correlating induced growth changes with changes in the content of any specific wall peroxidase isozyme. We recently characterized a monoclonal antibody, mWP3, which specifically crossreacts with a Mr 98,000 anionic peroxidase, which represents 15% of the total peroxidases present in soluble extracts of wall proteins from maize seedlings (10). Analysis by immunogold localization methods revealed t...

We examined the influence of short-term exposures of different UV wavebands on the elongation and phototropic curvature of hypocotyls of cucumbers (Cucumis sativus L.) grown in white light (WL) and dim red light (DRL). We evaluated (1) whether different wavebands within the ultraviolet B (UV-B) region elicit different responses; (2) the hypocotyl elongation response elicited by ultraviolet C (UV-C); (3) whether irradiation with blue light-enriched white light (B/WL) given simultaneous with UV-B treatments reversed the effect of UV in a manner indicative of photoreactivation; and (4) whether responses in WL-grown plants were similar to those grown in DRL. Responses to brief (1-100 min) irradiations with three different UV wavebands all induced inhibition of elongation measured after 24 h. When WL-grown seedlings were irradiated with light containing proportionally greater short wavelength UV-B (37% of UV-B between 280 and 300 nm), inhibition of hypocotyl elongation was induced at a threshold of 0.5 kJ m(-2), whereas exposure to UV-B including only wavelengths longer than 290 nm (and only 8% of UV-B between 290 and 300 nm) induced inhibition of hypocotyl elongation at a threshold of 1.6 kJ m(-2). The UV-C treatment induced reduction in elongation at a threshold of <0.01 kJ m(-2) for DRL-grown plants and <0.03 kJ m(-2) for WL-grown plants. B/WL caused 50% reversal of the short-wavelength UV-B-induced inhibition of elongation in DRL-grown seedlings but did not reverse the effect of long-wavelength UV-B. B/WL caused 30% reversal of the UV-C-induced inhibition of elongation in WL-grown seedlings but did not affect the response to short-wavelength UV-B. Short-wavelength UV-B also induced positive phototropic curvature in both types of seedlings, and this was reversed 60% or completely in DRL-grown and WL-grown seedlings, respectively. The similarity of responses between the etiolated (DRL-grown) and de-etiolated (WL-grown) seedlings indicates that the short-wavelength specific response may be relevant to natural light environments, and the apparent photoreactivation implicates DNA damage as the sensory mechanism for the response.