Canine distemper virus has been isolated in dog lymphocyte cultures from the brains of three javelinas that became moribund with signs of encephalitis. Canine distemper viral antigen was demonstrated predominantly in neurons and morbillivirus-like structures were seen by electron microscopy in brains of diseased animals. Serological studies suggest that CDV infection may be common in javelinas.

Infectious fibromas developed in eight domestic rabbits that were all kept in outside cages. Each rabbit had a single cutaneous tumor. Six of these tumors, which were studied by light microscopy, consisted of tumor cells resembling fibroblasts and large areas of inflammation. The overlying epidermis was hyperplastic and contained easily recognizable intracytoplasmic inclusion bodies. Inclusion bodies were also in subepithelial fibroma cells. Electron microscopy of three of these tumors revealed typical pox-virus inclusions, sites of viral replication, maturation, and mature particles in epithelial cells of the epidermis and in subepithelial tumor cells. Virus was not seen in inflammatory cells or leukocytes within vascular channels.

Abstract. Visceral leishmaniasis was diagnosed in a 2-year-old male Great Dane imported from Greece. The clinical signs included anemia, diarrhea, weight loss, proteinuria, and hyperglobulinemia with increases in a*-and y-regions. At necropsy there was plasmalymphocytic and macrophage infiltration of spleen, macrophage infiltration of bone marrow, intracellular organisms of macrophages having the ultrastructural characteristics of Leishmania species, and severe amyloidosis of the spleen, liver, and kidney.

Inclusion bodies occur frequently in the nuclei and rarely in the cytoplasm of osteoclasts in pigs with experimental lead poisoning. The light and electron microscope pictures of undemineralized sections are similar to those described for liver cord cells and renal tubular cells.

EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.

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One of two gray squirrels (Sciurus curolitierrsis) with typical skin lesions attributable to the squirrel fibroma virus also had generalized disease involving fibromatous reactions in the lung, liver, kidney, lymph node; and focal adenomatoid changes in the lung.

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