The kinetics of the secretion of CD40L are influenced by the method used to prepare PLTs for storage. The patterns for CD40L membrane association and secretion are different than those observed for CD62P during storage.
These studies indicate that CD34+ cell counts were maintained when CB samples were stored at 1 to 6 degrees C in test tubes for 3 days. Cryopreservation-thawing resulted in changes in a number of parameters including the percentage of CD34+ cells that were aldehyde dehydrogenase(+) and the number of 7-aminoactinomycin D(+) cells and SYTO16(low) cells.
These studies indicate that substantial variability in CD34+ cell levels were determined with flow cytometry. The variability in NC and MNC levels was minimal with HA methodology.
PBPCs can be maintained at 1 to 6 degrees C for up to 3 days and can be cryopreserved after extended storage with properties minimally altered. Dilution alone, without centrifugation and washing, of thawed PBPC samples is a satisfactory procedure for preparing samples for in vitro assays.
This study of intake procedures was initiated with a view towards reducing the early dropout rate of substance abusers. Eight different therapists conducted intake interviews of 634 cocaine dependent, first admissions to an outpatient cocaine treatment program. No significant differences in return rate were found across either the eight therapists or their level of academic training. We found that matching therapists and patients with respect to gender and race for the intake interview did not increase the proportion of patients returning for a second visit. In addition, the hypothesis that providing a sense of continuity by having the therapist who conducted the intake interview become the treatment therapist did not result in a higher return rate than if the patient was assigned to a different treatment therapist. Although the results do not indicate methods of further reducing early treatment dropouts they are helpful in reducing concerns about who conducts intake interviews and how patients are assigned.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.