Retention of cellular properties of PBPCs following liquid storage and cryopreservation

Abstract

PBPCs can be maintained at 1 to 6 degrees C for up to 3 days and can be cryopreserved after extended storage with properties minimally altered. Dilution alone, without centrifugation and washing, of thawed PBPC samples is a satisfactory procedure for preparing samples for in vitro assays.

Long‐term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer

“…The ability to store HSCs for short periods of time is desirable for several reasons and has been studied for a variety of HSC products 34‐41 . Because studies suggesting that holding HSC products for 24 hours should not be detrimental to postthaw recovery, cells frozen no more than 4 hours after collection were compared to cells stored for 24 hours before cryopreservation.…”

Short‐term liquid storage of CD14+ monocytes, CD11c+, and CD123+ precursor dendritic cells produced by leukocytapheresis

“…The mechanisms responsible for the variable loss of special WBC populations (CD14+, CD11c+, and CD123+ cells) remained unclear and should be subject to further studies. We recommend short‐term liquid storage of WBCs in autologous plasma as the method of choice especially in blood banks without technical equipment for cryoconservation 37 . Additional investigations should be performed on the in vivo function of MNCs and preDCs subsequent to WBC transfusion.…”

Flow Cytometric Human Leukocyte Antigen-B27 Typing with Stored Samples for Batch Testing

“…This suspension was then mixed with the same volume of cryosolution (6% hydroxyethyl starch, 4% fetal calf serum, 5% dimethyl sulfoxide in RPMI; final concentration) in ice water [15], and stored at -80℃. Just prior to testing, frozen MNCs were immediately thawed in thawing solution (2% fetal calf serum in RPMI).…”