Aim: The aim of present work was to determine the anticonvulsant and antioxidant activity of Senna occidentalis L. ethanolic seed extract by different models. Methods: For evaluation of anticonvulsant activity, Pentylenetetrazole (PTZ) seizure model and Maximal electroshock (MES) seizure model were used. For antioxidant activity, (1, 1-diphenyl - 2-picryl hydrazine (DPPH) and hydrogen peroxide (H2O2) method were used. Results: The finding suggested that the ethanolic extract (EAE) of Senna occidentalis in the dose 400 mg/kg body weight posses potent anticonvulsant activity. The EAE showed anticonvulsant action in dose dependent fashion. It was observed that upon increasing the concentration of extract, it showed reduced absorbance and increased free radical inhibition, and when comparison was made with Ascorbic acid, it showed marked antioxidant property in DPPH as well as H2O2 method. The IC50 of Ascorbic acid and EAE by DPPH method were found to be 14.56 and 14.8 respectively whereas the IC50 of Ascorbic acid and EAE by H2O2 method were found that 14.3and 14.8 respectively. Conclusion: The results of the present study concluded hat the EAE of Senna occidentalis L. possesses significant antioxidant and anticonvulsant activity. The activity was in dose dependent fashion. This study will assist in future research associated with formulation development of seeds of Senna occidentalis L. Keyword: Senna occidentalis L., Anticonvulsant, Antioxidant, DPPH model
Background: Sarasvata Churna is an Ayurvedic formulation for treatment and management of Epilepsy and other maniac disorders since thousands years. Objective: This study aimed to evaluate the in-vitro antioxidant potential, total phenolics and flavonoids content, acute-oral-toxicity and anticonvulsant activity of Sarasvata churna. Materials & Methods: In-vitro antioxidant activity of methanolic extract of Sarasvata churna against Nitrous oxide, Peroxide, Phosphomolybdenum and Hydroxyl radicals performed using Colorimetry against Ascorbic acid as standard along with estimation of total phenolic and flavonoids content. Acute oral toxicity was evaluated using OECD guidelines. Extract in carboxymethyl cellulose at doses of 50,75,100,125,150 and 200mg/kg was screened for anticonvulsant activity using subcutaneous Pentylenetetrazole and Maximal Electroshock models in Swiss Albino Mice (n=6). Sodium valproate was used as standard. Results: IC50 value of methanolic extract in the Nitrous oxide, Peroxide, and Hydroxyl free radical scavenging assay was found to be 165mg/ml, 32.5mg/ml and 253.9mg/ml respectively as compared to 61.58µg/ml, 333.44µg/ml and 351µg/ml respectively of standard Ascorbic acid. In acute oral toxicity screening, animals did not showed any signs of acute and delayed toxicity even up to a dose of 2000mg/kg. Extract offered a protection of 57.39% and 85.26% in scPTZ model (P<0.0001) and 74% and 96.38% in MES model (P<0.0001) at doses of 50 and 200mg/kg respectively as compared to standard at 95% Confidance interval (ANNOVA, Tukey test) indicating a dose dependent protection. Conclusion: Sarasvata churna’s potentials are comparable with standard antioxidant Ascorbic acid and antiepileptic drug Sodium valproate. This preclinical and toxicity screening data can be beneficial in establishing the scientific basis for the use of Sarasvata churna in management of epilepsy.
: Ayurveda prescribes the use of Medhya Rasayanas for the prevention and management of brain disorders like Epilepsy, Schizophrenia, Parkinson’s, and Alzheimer’s diseases. Medhya Rasayanas are a group of nootropic herbs which improves cognition, memory, intelligence, creativity, learning skills and executive functions. Additionally, these supplements also boost immunity. These herbs have intrinsic nature to exert these effects on the human brain. This review is an attempt to study and correlate the ethno-pharmacological basis of Medhya Rasayanas with their Antiepileptic potential. In epilepsy, Medhya Rasayanas either alone or in combination are prescribed to manage epileptic patients in Ayurveda. These herbs are time tested and also possess scientifically proven pharmacological effects. In this review, detailed pharmacognostical, phytochemical and pharmacological investigation on Medhya Rasayanas like Centella asiatica, Convolvulous pluricaulis, Glycyrrhiza glabra, Tinospora cordifolia, Acorus calamus, Bacopa monnieri, Celastrus paniculatus, Benincasa hispida, Nardostachys jatamansi, mineral like Swarna Bhasma (Gold Calyx) and few herbal formulations like Panchgavyaghrita, Brahmi Ghrita, Mentat, Asthamangal Ghrita, Sarasvata Churna, Sarasvata Arishta, and Samvardhan ghrita which contains Medhya Rasayanas was performed using various search engines like Google Scholar, Scopus, SciFinder, ScienceDirect, PubMed, Bentham Sciences and other online and print journals and scientific databases on medicinal plants. The rigorous review of the literature proves that each Medhya Rasayana is acting with different mechanisms of action to offer neuroprotection. The review strengthens the ethno-pharmacological claim of Medhya Rasayanas for their use in the treatment and management of Epilepsy and other brain disorders.
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