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This study evaluated the prevalence of nontuberculous mycobacterium (NTM) in relation to the total number of cases of mycobacterial infections detected in patients admitted at the University Hospital of Santa Maria from 2008 to 2010. From the positive samples for the genus Mycobacterium, 67% belonged to the Mycobacterium tuberculosis complex (MTBC) and 33% of them were classified as NTM. This investigation aims to contribute to the epidemiology of mycobacterioses, inasmuch as patients infected by NTM require distinctive treatment and monitoring in comparison with those infected by MTBC.

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This study aims to compare the frequency of Staphylococcus aureus nasal carriage among students from a Portuguese higher health school. Antimicrobial susceptibility testing was also assayed in order to detect methicillin-resistant S. aureus (MRSA) strains among the isolates. Nasal swabs and fingerprints from 60 healthy nursing and pharmacy students were collected, followed by inoculation and incubation at 37 degrees C for 24 h. All suspected S. aureus isolates were identified by routine laboratory procedures. The susceptibility to antimicrobial agents (tetracycline, gentamicin, chloramphenicol, amoxicillin/clavulanic acid, trimethoprim/sulphamethoxazole, oxacillin and vancomycin) of confirmed isolates was determined by a disc-diffusion method. Results showed 41.7% S. aureus colonisation among participants, and that the difference between nursing and pharmacy students was statistically significant. Antibiotic susceptibility testing demonstrated that S. aureus isolates showed variable sensitivity to antibiotics but, most importantly, were resistant to oxacillin and vancomycin. Although the frequency and prevalence of colonisation found is within the range previously described in healthy populations, increased resistance to antimicrobials and higher prevalence of MRSA among the student community was found.

Twenty-three isolates of Staphylococcus aureus resistant to methicillin have been analyzed, being found a clinical isolate of VISA through microdilution technique. The others techniques were unable to detect such isolates. This is the first study that shows the presence of VISA in clinical isolates in the city of Santa Maria-RS.

The study aimed to compare the effects of intraosseous infusion of lactated Ringer's and 0.9% sodium chloride solutions on the electrolytes and acid-base balance in pigeons submitted to humerus osteosynthesis. Eighteen pigeons were undergoing to isoflurane anesthesia by an avalvular circuit system. They were randomly assigned into two groups (n=9) receiving lactated Ringer's solution (LR) or 0.9% sodium chloride (SC), in a continuous infusion rate of 20mL/kg/h, by using an intraosseous catheter into the tibiotarsus during 60-minute anesthetic procedure. Heart rate (HR), and respiratory rate (RR) were measured every 10 min. Venous blood samples were collected at 0, 30 and 60 minutes to analyze blood pH, PvCO2, HCO3 -, Na+ and K+. Blood gases and electrolytes showed respiratory acidosis in both groups during induction, under physical restraint. This acidosis was evidenced by a decrease of pH since 0 min, associated with a compensatory response, observed by increasing of HCO3 - concentration, at 30 and 60 min. It was not observed any changes on Na+ and K+ serum concentrations. According to the results, there is no reason for choosing one of the two solutions, and it could be concluded that both fluid therapy solutions do not promote any impact on acid-base balance and electrolyte concentrations in pigeons submitted to humerus osteosynthesis.