Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.

2020) Development of silver nanoparticle-based hydrogel composites for antimicrobial activity, Green Chemistry Letters and Reviews, 13:1, 34-40, ABSTRACT Antimicrobial function of Ag nanoparticles (NPs) has a strong correlation with the released Ag + cations that are produced by oxidation of Ag NPs in a solution state under ambient condition. Therefore, in order to develop anti-infective materials for biomedical applications, one needs to include Ag NPs inside biocompatible materials, which can allow slow release of Ag + cations. Hydrogels of natural polymers could be an ideal choice for the purpose because (a) the physicochemical properties of hydrogels resemble with biological tissue, and (b) the inclusion of Ag NPs inside hydrogels prevents the direct release of Ag NPs, while allowing the release of Ag + cations out of the hydrogels. In this regard, we present a simple strategy for producing Ag NPscontaining hydrogel based on natural alginate polymers. The chemical modification of alginate, blending with Ag NPs, gelation by photo-crosslinking process have been discussed in connection with antimicrobial reaction on model bacterium.

Polydopamine was utilized an adhesive interface for the uniform coating of Au nanoparticles in the pores of polydimethylsiloxane to enhance the output performance of triboelectric nanogenerators.