Nigella sativa has been marked as precious medicinal herb consisting various bioactive compounds including Thymoquinone and very strengthen evidences can be found in literature in this regard. Nigella sativa has popularized as a miracle medicinal herb against pathogenic microorganisms including human infected deleterious viral species such as Human Immunodeficiency Virus (HIV) and Hepatitis C Virus (HCV). Recent animal studies have proved that, Nigella sativa seed extract can apply against virus species including Murine Cytomegalovirus (MCMV), Papaya Ring Spot Virus, Avian influenza (H9N2), Newcastle disease virus (NDV), Peste des Petits Ruminants (PPR) Virus. It is important to focus on Thymoquinone as main bioactive compound of the Nigella sativa. Among them, anti-cancer effect, cardiovascular effects, gastro-protective effect, anti-diabetic activity and free radicle scavenging activities are the most beneficial effects revealed in recent research studies. Hence, more discreet investigations required for the pharmacological actions of bioactive compounds of the Nigella sativa specially for the drug development.
Chemical speciation modelling in combination with potentiometric titration method was used to determine the protonation constants for 2-hydroxy-1-(2-hydroxy-4-sulpho-1-naphthylazo)-3-naphthoic acid, the Patton and Reeder's reagent, at 25° C in three ionic strengths of 0.1 mol dm-3 , 0.01 mol dm-3 and 0.001 mol dm-3 NaNO 3. Potentiometric titrations were performed and pH, titrant volume, temperature and free and total proton concentrations at each titration point were measured. Constants were determined for three of the four protonated sites and the forth one being a very low value as this site is always in the ionic form and is readily ionized in aqueous solutions.
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