The effects of salinity, temperature and pH on the activity in the spermatozoa of Siganus guttatus were studied. Milt were collected from 8 wild mature males that came from a commercial hatchery. Sperm motility was measured with a VHS video-recorder and a video camera connected to a microscope. The results showed that most of the sperm were highly motile in 400-700 mM NaCl, the longest duration of sperm motility recorded in 600mM NaCl and the duration was 788.0±16.9 s. Sperm was not motile when suspended at pH 4, sperm motility was observed at pH 5, there was a relatively higher percentage of motile sperm in solutions at pH 5-8 (above 80%), the duration was the longest and achieved (769.0±19.8) s when pH was 8. The motility and duration increased within 10-25ºC and decreased at the range of 25-40ºC, the highest motility and the longest duration were all appeared at 25 ºC, they were (91.0±1.4) % and (725.0±21.2) severally. Optimum salinity, temperature and pH for S.guttatus reproduction was 600mM NaCl, 25 ºC and 8 respectively.
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