Suppressive Effects of Copper Sulfate Accumulation on the Spermatogenesis of Rats

Liu, J. Y.; Yang, Xu; Sun, Xudong; Zhuang, Cuicui; Xu, Fengping; Li, Y. F.

doi:10.1007/s12011-016-0710-7

Cited by 32 publications

(22 citation statements)

References 33 publications

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“…The HPG axis of male is regulated by many factors. Exposure to environmental toxins may lead to changes in spermatogenesis and fertility [29]. In the present study, levels of serum T, FSH and LH at different sampling times after administration were measured and the results were shown in Figure 6(A-C).…”

Section: Changes In Sex Hormone Levelsmentioning

confidence: 79%

Systematic evaluation of CdSe/ZnS Quantum Dots toxicity on the reproduction and offspring health in male BALB/c mice

Lin

Chen

et al. 2020

Preprint

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Background: Increased applications of quantum dots (QDs) in biomedical field have aroused attention for their potential toxicological effects. Although many studies have been carried out on the toxicity of QDs, their effects on reproductive and development are still unclear. In this study, we systematically evaluated the male reproductive toxicity and developmental toxicity of CdSe/ZnS QDs in BALB/c mice.Results: The male mice were injected intravenously with CdSe/ZnS QDs at the dosage of 2.5 mg/kg BW or 25 mg/kg BW, respectively, and the survival status, biodistribution of QDs in testes, serum sex hormone levels, histopathology, sperm motility and acrosome integrity was measured on Day 1, 7, 14, 28 and 42 after injection. On Day 35 after treatment, male mice were housed with non-exposed female mice, and then offspring number, body weight, organ index and histopathology of major organs, blood routine and biochemical tests of offspring were measured to evaluate the fertility and offspring health. The results showed that CdSe/ZnS QDs could rapidly distribute in the testis, and the fluorescence of QDs could still be detected on Day 42 post-injection. QDs had no adverse effect on the structure of testis and epididymis, but high-dose QDs could induce apoptosis of Leydig cells in testis at an early stage. No significant differences in survival of state, body weight organ index of testis and epididymis, sex hormones levels, sperm quality, sperm acrosome integrity and fertility of male mice were observed in QDs exposed groups. However, the development of offspring was obviously influenced, which was mainly manifested in the slow growth of offspring, changes of organ index of main organs, and the abnormality of liver and kidney function parameters.Conclusion: Our findings revealed that CdSe/ZnS QDs were able to cross the blood-testis barrier (BTB), produce no discernible toxic effects on the male reproductive system, but could affect the healthy growth of future generations to some extent. In view of the broad application prospect of QDs in biomedical fields, our findings might provide insight into the biological safety evaluation of the reproductive health of QDs.

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Section: Changes In Sex Hormone Levelsmentioning

confidence: 79%

Systematic evaluation of CdSe/ZnS Quantum Dots toxicity on the reproduction and offspring health in male BALB/c mice

Lin

Chen

et al. 2020

Preprint

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“…Kowal et al found that the copper metabolism disorders could affect the sperm motility, sperm count, sperm head morphology, integrity of plasma membrane in tail cells, and the structure of testicular tissue [32]. In human and animal experiments, male gonads were particularly sensitive to the increase of copper concentration, which leads to the decrease of testosterone level, the degradation of spermatogenic tubule epithelium, the decrease of sperm count and motility, the inhibition of acrosome reaction and spermatogenesis , and the increase of cell apoptosis, so that the fertility of the male was reduced [29][30][31][32][33][34][35]. ATP7A could protect tissue from excessive copper and played a crucial role in cellular copper homeostasis [36,37].…”

Section: Discussionmentioning

confidence: 99%

Proteome Analysis of Testicular Tissue in Varicocele Rats Using iTRAQ Labeling Technology

feng

Zhao

rong

et al. 2020

Preprint

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Purpose Varicocele (VC) is considered as the main cause of male infertility, clear and definite molecular markers of varicocele disease are helpful for early prevention and timely treatment. This study was in order to examine the effect of varicocele on protein expression in testicular tissue.Methods The testicular tissue samples of normal rats and varicocele rats were used for proteomic analysis and functional bioinformatics analysis. The proteins with a fold change of > 1.2 or < 1.2, and with a P-value < 0.05 were used to identify up- and downregulation proteins between the varicocele and control rats, and two target proteins were selected and verified with western blot.Results It was found that seminiferous epithelium was disordered and spermatogenic cells were injured seriously in varicocele group. A total of 65 differentially expressed proteins were identified compared with normal group by liquid chromatography tandem mass spectrometry (LC-MS/MS) and isobaric tags for relative and absolute quantitation (iTRAQ) analysis, including 31 up-regulated proteins and 34 down-regulated proteins, respectively. Functions of those proteins were mainly related to the following processes: signal transduction, protein cycle, transmembrane transport, protein transport, vesicular mediated transport, cell division, and membrane tissue. Two down regulated proteins of ATPase and Cu2+-transporting alpha (ATP7A) and Calcium and integrin-binding protein 1 (CIB1) in varicocele rats were selected and confirmed.Conclusion Protein spectrum of testicular tissue in varicocele rats was different from that in normal rats. Low expressions of ATP7A and CIB1 may be affect testicular spermatogenic function and can be used as a potential biomarker for testicular tissue to maintain normal spermatogenic function.

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“…The both of applications were made on the same day simultaneously. The doses of Cu and propolis were determined according to previous researches (Ahmed et al, 2017;Liu et al, 2016;Tatli Seven et al, 2018;Zhang, Noordin, Rahman, & Haron, 2000).…”

Section: Experimental Designmentioning

confidence: 99%

Bee glue (propolis) improves reproductive organs, sperm quality and histological changes and antioxidant parameters of testis tissues in rats exposed to excess copper

Seven

Baykalır

et al. 2020

Andrologia

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This study was designed to determine the effects of propolis on the sperm quality, antioxidant and histological parameters in the testicular tissues of male Sprague Dawley rats exposed to excessive copper (Cu). In this aim, 24 rats were randomly divided into four groups as follows: the control, Cu, Propolis and Cu+Propolis. When compared to control group, Cu administration significantly decreased sperm motility and concentration, increased total abnormal sperm rate. It caused a significant induction the malondialdehyde (MDA), and reduction the superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) in testicular tissues. Also, it caused loss, disorganisation and vacuolation of the germinal epithelium, oedema of the interstitial tissues, proliferation of the interstitial cells, spilled immature spermatogenic cells in the lumen of some seminiferous tubules. A large number of active caspase‐3‐positive stained apoptotic cells and a significant decrease in Johnsen's testicular score were determined. However, significant ameliorations were observed in all sperm characteristics, MDA, SOD, CAT, GSH, seminiferous tubules, number of apoptotic cells and Johnsen's testicular score in Cu+Propolis group. Our results showed that oral supplementation of propolis had curative effect on the sperm quality, antioxidant and histological parameters in the testicular tissues of male Sprague Dawley rats exposed to Cu.

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Suppressive Effects of Copper Sulfate Accumulation on the Spermatogenesis of Rats

Cited by 32 publications

References 33 publications

Systematic evaluation of CdSe/ZnS Quantum Dots toxicity on the reproduction and offspring health in male BALB/c mice

Systematic evaluation of CdSe/ZnS Quantum Dots toxicity on the reproduction and offspring health in male BALB/c mice

Proteome Analysis of Testicular Tissue in Varicocele Rats Using iTRAQ Labeling Technology

Bee glue (propolis) improves reproductive organs, sperm quality and histological changes and antioxidant parameters of testis tissues in rats exposed to excess copper

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