In this paper, we describe a method for detecting and correcting in-plane bulk translational motion in multislice spin-echo imaging using self-calibration and postprocessing. A constant phase encoding offset between slices is used to evenly spread out the low spatial frequency echoes to allow accurate motion detection by self-calibration. Motion detection in both x and y directions is achieved by interchanging the readout and phase encoding directions for the alternate slices. Displacements are determined by cross correlating the modulus of each 1D transformed echo with a reference box car function whose width equals that of the imaged object. In addition, phase errors induced by the velocity in the readout direction are estimated and corrected using the displacement data. The results obtained from knee studies at 0.5 T and 1.5 T show that the artifacts due to translational motions are significantly suppressed upon correction. The method does not require any additional pulses or time, and the data processing can be easily implemented.
Electron tomography is an important tool for electron microscopists trying to understand the 3-dimensional structure of their specimens. Many TEM specimens, particularly biological ones, are sensitive to the total electron dose used during imaging. Several groups have demonstrated procedures for minimizing the dose during electron tomography [1,2,3,4]. Here we describe an approach to minimizing the electron dose for use with the Gatan TEM Tomography Software [5]. By offsetting the tracking areas from the data acquisition area the electron dose on the specimen is reduced by a factor of 4. In addition to the low-dose capability, this solution is supported on a broad range of microscopes from different vendors, is fully integrated into the Gatan Microscopy Suite, and benefits from the image processing capabilities and other tools that this provides.The acquisition of a tomographic tilt series would be straightforward were it not for the mechanical limitations of a typical microscope stage. As a specimen is tilted from one angle to the next the goniometer imposes both random and systematic shifts in X, Y, and Z on the specimen. These shifts can cause a loss of focus or lose the field of interest after a small number of tilts, depending on the magnification and the properties of the given stage.Gatan's tomography software allows automated and pre-calibration based methods to correct for stage errors [1]. In the automated method, drift in the X-Y plane is measured by comparing an image from the current tilt angle to a reference image. To measure the drift in the Z direction the beam-tilt induced image-shift method is used [6].Here we describe an extension to the Gatan TEM Tomography Software that allows the user to define regions for automated X/Y and Z tracking that are displaced along the tilt axis from the data acquisition area. Each of the tracking regions and the data acquisition area can have different illumination conditions (spot size, brightness). The electron dose in the tracking regions can be increased over that in the data acquisition area in order to improve the signal-to-noise ratio of the images used in determining the drift and hence improve the reliability of these measurements.A graphical user interface is provided to help set up the automated tracking regions. The user adjusts the relative offsets of the two tracking regions from the data acquisition region by means of sliders ( Figure 1) and can record the illumination conditions to be used at each of the 3 regions. The software assists the user by constraining the region's offset to lie on the tilt axis and allows the user to preview a sample image from any of the 3 regions (Figure 2).For users with electron-dose sensitive specimens the ability to acquire tracking data from regions other than the area of interest should enable acquisition of tilt series from specimens that would otherwise suffer too much damage to be useful or track with a higher signal-to-noise ratio than before while still reducing the overall electron dose on the area of inter...
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