Squalene in food and feed undergoes polymerization and autoxidation. At conditions of thiobarbituric acid (TBA) method with alkaline hydrolysis in water solution of 0,35 N NaOH during 5 min and distillation with steam the oxidized squalene liberates malonic dialdehyde like oxidized unsaturated fatty acids of lipids.
Optimum parameters have been determined for separation and determination of malonic dialdehyde from oxidized squalene. Dependent on oxidation conditions squalene can undergo considerable oxidation in limits from 0 to value 1008 in TBA numbers.
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