Tobacco yellow dwarf and bean summer death diseases apparently are absorbance typical of a nucleoprotein, contain a single centrifugal confined to Australia, and the. causal pathogens are transmitted •by the component with a sedimentation coefficient of 76S, and yield a single leafhopper Orosius argentatus. The diseases were shown to be caused by structural protein species of molecular weight 27,500. The yield of TYDV viruses. Purified preparations obtained from Datura stramonium plants, was about 100-250 Ag/ kg of tissue, and of BSDV about 20-50 jug/ kg of infected with either tobacco yellow dwarf virus (TYDV) or bean summer tissue. In addition to producing similar host reactions and having similar death virus (BSDV), contained viruslike particles occurring in pairs about vector relations, TYDV and BSDV are serologically related, and are 20 nm X 35 nm in size. On sedimentation in sucrose density gradients, the therefore considered likely to be strains of one virus. TYDV and BSDV geminate particles of TYDV and BSDV each formed discrete zones which have many properties in common with beet curly top virus (CTV), and a were associated with infectivity. TYDV preparations have an ultraviolet distant serological relationship was established between TYDV and CTV.
SUMMARY
Two strains of a virus designated Glycine mosaic virus (GMV) were found in Glycine clandestina and G. tabacina, legumes indigenous to Australia and the western Pacific region. When transmitted by sap inoculation, GMV infected mostly leguminous species, and caused mosaic and mottling symptoms. The virus was not found naturally in soybean G. max, but it infected all of the 21 cultivars tested.
GMV has isometric particles of c. 28 nm diameter, and produces three components with sedimentation coefficients of 60 S (top), 103 S (middle), and 130 S (bottom). Both middle and bottom components are required for infectivity. The virions contain two major proteins with molecular weights of c. 21 500 and 42 000. GMV produces large aggregates of particles in the cytoplasm of the mesophyll cells of pea Pisum sativum, and also induces amorphous membrane‐bound bodies and cytoplasmic vesicles. The type strain (from New South Wales) reacts with antisera to Echtes Ackerbohnenmosaik, broad bean stain, and a Californian isolate of squash mosaic virus. The GW strain (from Queensland) reacts with all of the latter antisera, as well as with antisera to cowpea mosaic virus (Sb and Ark strains), bean pod mottle, and red clover mottle viruses, and is serologically related to, but not identical with, the type strain. These properties clearly establish GMV as a new member of the comovirus group.
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