We present an experimental study of the self-assembly of capsid proteins of the cowpea chlorotic mosaic virus (CCMV), in the absence of the viral genome, as a function of pH and ionic strength. In accord with previous measurements, a wide range of polymorphs can be identified by electron microscopy, among them single and multiwalled shells and tubes. The images are analyzed with respect to size and shape of aggregates, and evidence is given that equilibrium has been achieved, allowing a phase diagram to be constructed. Some previously unreported structures are also described. The range and stability of the polymorphs can be understood in terms of electrostatic interactions and the way they affect the spontaneous curvature of protein networks and the relative stabilities of pentamers and hexamers.

The self-assembly of many viral capsids is dominated by protein-protein electrostatic interactions. To have a better understanding of this process, it is important to know how the protein and the capsid surface charges vary as a function of the pH and ionic strength. In this work, using phase analysis light scattering, we measured the electrophoretic mobility (EM) of the cowpea chlorotic mottle virus (CCMV), its capsid protein (CP), and a cleaved CP that lacks its basic terminus, as a function of pH and ionic strength. The EM measurements of the CP are difficult to carry out due to its tendency to self-assemble into capsids; we show how to circumvent this problem by appropriately changing the CP concentration. We found that the isoelectric points (pIs) of the virion and of the CP are insensitive to ionic strength. The onset of multishell structures in the phase diagram of the CCMV CP as a function of ionic strength and pH (and its absence in the brome mosaic virus (BMV) CP phase diagram) can be related to the pI of the capsid. We propose that the transition from multiwall shells to nanotube structures is due to a change in the spontaneous curvature of the CP at its pI. A nonzero limit of the EM at high ionic strength is characteristic of a soft colloid, but a near identity of the EMs of empty capsids and those containing RNA indicates that the EM reflects only the charge distribution in the CP. The Henry equation has been used to provide approximate values of the capsid surface charge as a function of pH and I.

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(2017) Preparation of chitosan matrices with ferulic acid: physicochemical characterization and relationship on the growth of Aspergillusparasiticus, CyTA -Journal of Food, 15:1, 65-74, DOI: 10.1080/19476337.2016 To link to this article: https://doi.org/10. 1080/19476337.2016 Antioxidant, anticarcinogenic, and antimicrobial properties have been reported for ferulic acid (FA), therefore, its application interests both food and agriculture research. FA was immobilized in different chitosan (CS) matrices, physicochemicaly characterized and the effect on Aspergillus parasiticus ecological parameters evaluated. Nanoparticles (Nparticles), microparticles (Mparticles) and microcapsules (Mcapsules) of 35-40 nm, 30-40 μm, and 20 μm, respectively were obtained; FA incorporation in matrices affected their morphology, physicochemical properties, and their fungistatic effect. The effect of the particles was dependent on the matrix exposed. Nparticles and Mparticles showed high FA immobilization efficiency as well as a good fungistatic effect against A. parasiticus: Radial growth at 168 h was 28.46 ± 1.01 and 28.84 ± 1.36 and the inhibition of spore germination at 30 h was 57.44 ± 0.22 and 55.74 ± 2.19, for Nparticles and Mparticles, respectively compared with control cultures. Abnormalities in mycelium, hyphae, and spores morphology were observed, as well as low sporulation due particle interaction with the fungus.Preparación de matrices de quitosano con ácido ferúlico: Caracterización físicoquímica y su relación en el crecimiento de Aspergillus parasiticus RESUMEN El ácido ferúlico (AF) presenta propiedades antioxidantes, anticancerígenas y antimicrobianas, siendo de interés para su aplicación en alimentos y agrícultura. El AF fue inmovilizado en diferentes matrices de quitosano, se caracterizó fisicoquímicamente y se evaluó su efecto sobre Aspergillus parasiticus. Se obtuvierón nanopartículas, micropartículas y microcápsulas de 35-40 nm, 30-40 µm y 20 µm, respectivamente. La incorporación de AF en matrices afecto su morfología, propiedades fisicoquímicas y su efecto fungistático, dependiendo de la matriz expuesta. Las nano y micropartículas mostraron gran eficiencia de inmovilización de AF y efecto fungistático sobre el hongo: inhibición de crecimiento radial a 168 h de 28,46±1,01 y 28,84±1,36 y de germinación de esporas en 30 h de 57,44±0,22 y 55,74±2,19, respectivamente. Asimismo se observaron anomalidades en micelio y morfología de hifas y esporas, así como baja esporulación, debido a la interacción entre las partículas con el hongo. Las microcápsulas no ejercieron efecto antifúngico, sin embargo, tanto las nano-como las micro-partículas son buenos candidatos para el control de hongos.

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