No abstract

In a factorially designed experiment (N = 321), 0, 800 or 1600 I.U. pregnant mare serum gonadotrophin (PMSG) were administered in combination with 0, 12 or 18 mg follicle stimulating hormone (FSH-P) to superovulate Merino ewes in autumn and spring. A moderate dose of PMSG (800 I.U.) in conjunction with 12 or 18 mg FSH-P increased the ovulation rate above that observed when FSH-P was used alone. This was accomplished by (i) increasing the proportion of ewes that exhibited a superovulatory response (greater than 3 corpora lutea (CL) or persistent large follicles (LF): 69/70 (99%) v. 55/74 (74%), P less than 0.001), and (ii) in those ewes that exhibited a superovulatory response, by an additive effect of exogenous gonadotrophin (14.8 +/- 0.9 CL (69) v. 11.3 +/- 0.9 CL (55), P less than 0.01) without increasing the incidence of LF. The use of 1600 I.U. PMSG in conjunction with 12 or 18 mg FSH-P was characterized by an increase in the number of LF and, in comparison with 800 I.U. PMSG, a reduction in ovulation rate. Season had no effect on the numbers of CL, but total ovarian response (CL + LF) was higher in autumn than in spring (P less than 0.01), because of a greater incidence of LF (P less than 0.001). The proportion of ewes with regressed CL was higher in autumn than in spring (53/143 (37%) v. 32/156 (21%), P less than 0.01), and increased with increased dose of gonadotrophin. Furthermore, a nutritional component to the incidence of ewes with regressed CL was suggested by the observation that the mean concentration of plasma glucose was higher for ewes with normal CL than for ewes with regressed CL (P less than 0.05). Rates of ova or embryo recovery, fertilization and embryo development generally declined with an increase in the incidence of LF as a result of increases in the dose of gonadotrophin and season of administration.

No abstract

There has been considerable research on techniques for artificial insemination of sheep with frozen ram semen (Maxwell, 1984). Acceptable pregnancy rates were reported following ‘two-step’ dilution and freezing of semen in P.V.C. ‘ministraws’ (Colas, 1975; Colas & Guerin, 1981); however, other workers have obtained poor fertility following cervical insemination with semen frozen in straws (Maxwell, etal. 1980; Tervit elal. 1984).

An experiment examined the effects of treatment with gonadotrophin releasing hormone (100 micrograms GnRH injected 24 h after progestagen sponge removal), season of treatment (autumn v. spring), the effect of supplementary feeding with lupin grain (in autumn only, from 12 days before until 8 days after sponge removal) on the time of ovulation in 182 mature Merino ewes superovulated with a combination of 400 I.U. pregnant mare serum gonadotrophin (PMSG) and 12 mg follicle stimulating hormone (FSH-P). Time of ovulation was influenced by both season and the administration of GnRH. Two peaks of ovulation were observed in spring, the first between 24 and 30 h after sponge removal, and the second commencing 42 h after sponge removal. In autumn, there was one peak of ovulation between 42 and 54 h after sponge removal. Treatment with GnRH advanced the time of ovulation in autumn (P less than 0.05), but had no apparent effect in spring. In contrast to the effect of GnRH, time of ovulation for ewes treated in autumn was delayed (P less than 0.001) by supplementary feeding with lupins.