Germinated fenugreek seeds were separated into endosperm, seed coat and sprouts with an overall yield of 78%. Germinated endosperm, sprouts and ungerminated endosperm contained 39.25%, 36.12% and 48.20% protein, respectively. Germinated seed coat had 55.80% insoluble and 86.96% total dietary fibre, whereas ungerminted seed coat had 31.90% and 77.10% respectively. Sprouts were rich in polyphenols (97.55 mg per 100 g). At 200 ppm concentration, the antioxidant activity of the extracts of seed coat, sprouts and endosperm from germinated fenugreek seeds was 79.87%, 49.05%, and 13.42% where as the seed coat and endosperm of ungerminated fenugreek exhibited 90.94% and 10.13% antioxidant activity respectively. The GC analysis of the ester derivatives of the fixed oils of above fractions showed discernable changes in fatty acid profile. Atomic absorption spectroscopy analysis of the above fractions showed overall differences in their mineral content particularly calcium, zinc, copper, iron, potassium, manganese and magnesium.
The steam-distilled volatile oil of the large cardamom (Amomum subulatum Roxb.), grown in Sikkim, India, was analysed by GC-MS. It consists of monoterpenic hydrocarbons (16.3%), oxygenated rnonoterpenes (75.2%) and sesquiterpenes (6.3%). Its major constituents are l,&cineole (61.3%), a-terpineol, a-and P-pinene and alloaromadendrene. Twenty-five compounds are reported for the first time.KEY WORDS large cardamom; Amomum subulatum Roxb.; Zingiberaceae; steam-volatile constituents; mono-and sesquiterpenes; GC-MS
At present, the commonly used HPLC method for the analysis of caffeine
content in tea brews
employs direct application of the samples on the column. This
practice gradually reduces the
efficiency of the column and shortens its life. In the modified
method, the interfering tea pigments
are effectively removed by passing the sample through a Sep-Pak
C18 cartridge. Then its injection
on a reversed phase μ-Bondapak C18 column employing
acetonitrile and water (20:80 v/v) as mobile
phase reduces the analysis time without affecting either the resolution
of the peaks or the accuracy
of caffeine determination. This method is shown to estimate
accurately soluble caffeine contents
in the brews of black tea, decaffeinated tea, and decaffeinated instant
tea samples. Thus, the method
is ideally suited for rapid routine anaylsis of black tea and its
products.
Keywords: Caffeine; Sep-Pak C18; decaffeinated tea; HPLC;
instant tea
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.