Background: Cancer metastasis is the most important cause of cancer death and different treatment strategies have targeted on preventing the occurrence of metastasis. Quercetin is a flavonoid and widely used as an antioxidant and recent studies have discovered its anticancer and antiproliferative capabilities. Rac1 is protein known to involve in tumor invasion and metastases. Objectives: In this study, we investigated the effect of quercetin on expression of Rac1 protein. Materials and Methods:In this experimental study, HeLa cells were treated with quercetin at 2 concentrations (20 and 40 μM) for 24 hours and then cell lysate was assessed by Western blotting analysis to investigate the impact of quercetin on expression of Rac1 protein.Results: Quercetin significantly decreased Rac1 expression of HeLa cells assessed by western blotting, in dose-dependent manners (P < 0.05). Conclusions: This study showed that quercetin decreased Rac1 expression as a marker of metastasis in cervical cancer cells. Therefore, the quercetin may provide an effective new strategy to reduce of tumor metastasis.
Objectives: The present study aimed at evaluating the effects of aerobic exercise training (ET) and different doses of berberine chloride (BC) supplementation on apoptotic indices in renal tissue of streptozotocin (STZ)-induced diabetic Wistar rats. Methods: In the current study, 64 Wistar rats were divided into two normal and diabetic groups and then the normal group was divided into two subgroups including normal control (NC) and normal exercise training (NET, 3 d/wk, 50%-55% of VO2max for six weeks) and the diabetic group was divided into six subgroups including diabetic control (DC), diabetic exercise training (DET), diabetic BC15 (DBC: 15 mg/kg), diabetic BC30 (DBC: 30 mg/kg), diabetic BC15+ET (DBC15+ET), and diabetic BC30+ET (DBC30+ET). Intraperitoneal injection of STZ (100 mM citrate buffer, pH 4.5 contained 60 mg/kg STZ) was used for diabetes induction. Results: There was no significant difference in the levels of caspase-3 and Bax protein between the NC and DC groups (P = 0.59 and P = 0.49, respectively). The diabetic rats treated with BC30+ET demonstrated a reduction in caspase-3 compared with NC, DC, DBC15, and NET groups (P = 0.001, P = 0.02, P = 0.001, and P = 0.04, respectively). The results also revealed no significant difference in Bcl-2 protein level after six weeks of ET and BC supplementation in renal tissue of the studied groups (P = 0.33). Conclusions: These results suggested that aerobic exercise training combined with higher doses of BC could decrease pro-apoptotic indices with an increase in Bcl-2 to Bax ratio and suppress apoptotic cell death in the renal tissue of diabetic rats.
Objective: Insulin resistance (IR) is the major cause in Type 2 diabetes mellitus (T2DM). Expression of some miRNAs can be changed in response to a drug treatment for IR, and used as the biomarker in IR. This study set out to determine the effect of cinnamon extract (cinnamaldehyde) on some miRNAs expression in IR adipocytes. Materials and Methods: In this In-vitro study the 3T3L1 cells were expanded in Dulbecco’s modified Eagle’s medium (DMEM), differentiated into adipocytes phenotype and insulin resistant with high glucose medium, then the cells were treated with cinnamaldehyde. To determine of the miRNAs profiling in 3T3L1 adipocytes, insulin-resistant adipocytes and treated insulin-resistant adipocytes quantitative real-time PCR method was performed. Results: IR adipocytes exhibited a significantly increase in miRs 29a, 223 and 320 expression, and decrease in miR26-b expression in compare to the normal adipocytes (P-value<0.001 and P-value= 0.024 respectively). However in response to cinnamaldehyde in IR adipocytes, expression of miRs 29a, 223 and 320 were down-regulated while expression of miR26-b was up-regulated neared it to the normal level (P-value= 0.003 and P-value= 0.002 respectively). Conclusion: IR changes expression of intended miRs, so that cinnamaldehyde treatment helps to improve and normalize the changes. Cinnamon as the herbal product can be helpful for IR particular in adipose tissue.
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