CASTRATION IN GUINEA FOWLS 653 tablets were given alone. There were variations both above and below the average curve obtained in the series which had received the Armour tablets alone, but the majority of the determinations as well as the average curve are somewhat higher in the series in which the KI was administered in combination with the Armour anterior pituitary tablets.Twelve guinea pigs were used in a series of experiments in which thyroid substance was administered alone in doses of 0.05 grain (Armour thyroid substance) and in combination with KI 0.05 gtn. Both drugs were administered daily by mouth. The average curve of the metabolic rates in the set of 6 guinea pigs in which the thyroid substance was given alone is very similar to that obtained when Armour anterior pituitary tablets were administered. There is a fairly sudden rise within the first 8 to 12 days which ranges from 35 % to 50% above the normal. This rise is followed by a fall which occurs in all the animals and ranges from 30% to 100%. After this there is a gradual and steady rise so that at the end of the 28 day period in which the animals were observed, the basal metabolic rate reaches a level approximately 50% above the normal rate. The animals which had received KI in combination with the thyroid substance showed in general a similar average curve of the metabolic rate to that obtained in those which had received the thyroid substance alone. There were variations in the determinations in the former series both above and below those in the latter series but the majority of the determinations as well as the average curve in the guinea pigs which had received thyroid substance with KI were slightly higher, especially in the latter periods, than in those which had received the thyroid substance alone.
Verzir and his collaborators" observed that the extracts of wheat-germs containing vitamin E, when applied intraperitonedly or subcutaneously, bring about the same biological reaction as the hormone from the anterior lobe of pituitary gland, i. e., oestrus and hypertrophy of the uterus (based on precocious activation of ovaries).The purpose of our experiments was to reproduce Verzir's results and thus to use this phenomenon as a convenient means of standardization of vitamin E preparations,* and further to compare the activity of vitamin E with that of pituitary hormone and prolan A. Three active vitamin E preparations were used, the potency of which was proved by the Evans-Burr method.For testing vitamin E preparations ( I and 11) 3 groups (6 in each) of infantiie female rats (30-35 days) were used. The first and second groups received daily intraperitoneally, for 14 days, 0.3 cc. of vitamin E preparations Nos. I and 11, respectively. The third group serving as control received the same amount of olive oil. Experimental and control animals gained in body weight and seemed healthy. No external signs of stimulation of sex development s x h as hypertrophy and hyperemia of labiae waginalis and opening of vagina were observed. On dissection also we found no signs of maturity, i. e., neither ripening of follicles nor hypertrophy and hyperemia of uterus and tubes.Similar tests were made with vitamin E preparations No. IIIa and IIIb. Three animals of the first group, and 2 of the second died after receiving 5 doses (0.3 cc.) while the other animals survived as many as 12 doses each. On the fifth day the animals were examined, killed and dissected. The experimental animals lost much weight. the skin of abdominal regions was wet, and the ab-1 Szarka, A.
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