For the first time since the introduction of female sex hormone treatment of carcinoma of the prostate, an entirely new therapeutic approach was tried, i.e., specific immune therapy in the form of chessboard vaccination. With this method, and continuation of hormone therapy, inactivated cancerous cells were injected with neuraminidase intracutaneously into the thigh. Should hormone therapy prove ineffective, a long-term therapeutic effect can be achieved in approximately half of the patients by giving three vaccinations containing 44,400,000 cells. The effectiveness of specific immunotherapy in the form of chessboard vaccinations could be established on the basis of a significant drop in serum phosphatases and carcinoembryonic antigen under therapy, the scintigraphic evidence of remission of osteometastases and not least of all a statistically significant increase in the survival rate during treatment of unconfined metastasizing prostate carcinomas. Even in early stages specific immunotherapy constitutes a new alternative therapeutic method, particularly in the treatment of hormone-independent prostate carcinomas and in cases of estrogen intolerance.
We have compared a series of 16 scalpel partial resections with a series of 17 CO2 laser resections in dog kidneys. In both groups, almost equal loss of function was found (PAH and Inulin clearance, isotopes, roentgenological checks). Subsequent suturing is necessary, because heavy bleeding occurs like that following scalpel incision.
Cytologic examinations of kidneys, which were frozen for preservation in a nitrogen atmosphere below 3 ata, show a considerable damage of the cells. This does not only occur by significant reduction in the amount of isolated cells, but also in the loss of the ability to form cell colonies. DNA measurements show, however, that after 3–4 days the DNA amounts in surviving cells are intact. In one of the cultures we even found DNA values, which stand for a DNA synthesis. Experiments with mechanical perfusion alone show that this procedure results in a severe damage of the cells, only about one-third of these cells being able to form colonies.
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