The fatty acids and mycolic acids of 16 clinical isolates of Mycobacterium malmoense were studied by gas chromatography and thin-layer chromatography. All strains contained 2-methyleicosanoic and 2,4,6-trimethyltetracosanoic acids and alpha-, alpha'-, and keto-mycolic acids. The reported findings suggest that lipid analysis is a very useful approach in the species identification of M. malmoense.
The fatty acids of 40 strains, representing 24 species, of rapid‐growing mycobacteria were analysed by gas chromatography and mass spectrometry. Tuberculostearic acid and straight‐chain fatty acids, ranging from 14 to 24 carbon atoms, were detected in all studied strains. Some strains were found to contain 2‐methyl branched‐chain acids: 2‐methyl‐2‐octadecenoic acid was identified in Mycobacterium fortuitum, 2,4‐dimethyl‐2‐eicosenoic acid in M. smegmatis and 2,4‐dimethyl eicosanoic acid in M. tokaiense.
We studied the release of mycobacterial fatty acids (as methyl esters) and secondary alcohols after heating both wet and freeze-dried cells in methanolic hydrogen chloride for different time periods. A 30-min heating of the mycobacteria without prior freeze-drying was found adequate in a routine gas chromatographic procedure for strain and species characterization.
Sixteen strains of Mycobacterium xenopi were studied by gas chromatography and thin-layer chromatography. Data on the cellular fatty acids, fatty alcohols, mycolic acids, and glycolipids indicated that this bacterium possesses a specific lipid composition. 2-Docosanol, detected in all studied strains, was found to constitute a useful chemical marker in the identification of M. xenopi.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.