I'he ;inticoagulant action of the heparin antagonist Polybreile has been studied in vitro. Eblybrene was fouild to interfere with the formation of intrinsic blood thrombc~plastin. On exarrlination of the steps in the forrnation of throinboplastin, there was no iilterferer~ce with the production of the intermediate, product I. As this intermediate product is forrned by the interaction of aluminium hydroxide ;idsorbed plasma, serurn, and calcium, Polybrene does not appear to interfere with the interaction of factors VIII, IX, XI, or X11. I t was noted, however, that the further reactions of product 1 with phospholipid were inhibited by this agerat. It is siaggested that the anticc~agulant action of E'olybrene is exerted a t this point ,~n d is dependent on its capacity to neutralize the surface charge of the phosphoiipid particles. (b) Thrombin (bovine 1000 N.I.N. unitsjml; Parke-Davis & Co.) diluted in ,\iP//8O CaC'12. (c) I'olybrenc (hexadinlcthrine broinide 1%; Abbott Laboratories) dilutecf in 0.97; saline. This poIylner was thought t o have a nlolec~~lr-tr weight of about 6000, although recently this has been revised t o approxi~nately %SOU (5). (d) ('itrated hunran plasma prepared from blood collected in 3.80 sodium citrate (one part t o nine parts of blood). (e) Brain throrllboplastin, an acetone extraction of human brain; cephalin, a chloroform extraction of brain thromboplastin; and veronal bufkr (pll 7.45). A411 were prepared as described by Biggs and Riacfarlane (ti). C:~nadian J o i~r~a ! of I'hysiology and Pharmacology. Volume 43 (1965) Can. J. Physiol. Pharmacol. Downloaded from www.nrcresearchpress.com by University of Tennessee on 12/27/14For personal use only.
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