The Anticoagulant Action of Polybrene

Zipursky, Alvin; Wodzicki, Agnes M.; Russet, J I; Israels, Esther D.; Israels, L. G.

doi:10.1139/y65-028

Cited by 5 publications

(2 citation statements)

References 2 publications

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“…As previously demonstrated, polybrene has an anticoagulant activity. 19 Plasma, was separated by centrifugation for 15 min at 2000 g at 4 C and immediately stored at À80 C until use.…”

Section: Binding Studies With Surface Plasmon Resonance (Spr)mentioning

confidence: 99%

Pharmacological inhibition of mannose-binding lectin ameliorates neurobehavioral dysfunction following experimental traumatic brain injury

Blasio

Fumagalli

Longhi

et al. 2016

J Cereb Blood Flow Metab

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Mannose-binding lectin is present in the contusion area of traumatic brain-injured patients and in that of traumatic brain-injured mice, where mannose-binding lectin-C exceeds mannose-binding lectin-A. The reduced susceptibility to traumatic brain injury of mannose-binding lectin double knock-out mice (mannose-binding lectin) when compared to wild type mice suggests that mannose-binding lectin may be a therapeutic target following traumatic brain injury. Here, we evaluated the effects of a multivalent glycomimetic mannose-binding lectin ligand, Polyman9, following traumatic brain injury in mice. In vitro surface plasmon resonance assay indicated that Polyman9 dose-dependently inhibits the binding to immobilized mannose residues of plasma mannose-binding lectin-C selectively over that of mannose-binding lectin-A. Male C57Bl/6 mice underwent sham/controlled cortical impact traumatic brain injury and intravenous treatment with Polyman9/saline. Ex-vivo surface plasmon resonance studies confirmed that Polyman9 effectively reduces the binding of plasma mannose-binding lectin-C to immobilized mannose residues. In vivo studies up to four weeks post injury, showed that Polyman9 induces significant improvement in sensorimotor deficits (by neuroscore and beam walk), promotes neurogenesis (73% increase in doublecortin immunoreactivity), and astrogliosis (28% increase in glial fibrillary acid protein). Polyman9 administration in brain-injured mannose-binding lectin mice had no effect on post-traumatic brain-injured functional deficits, suggestive of the specificity of its neuroprotective effects. The neurobehavioral efficacy of Polyman9 implicates mannose-binding lectin-C as a novel therapeutic target for traumatic brain injury.

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“…As previously demonstrated, polybrene has an anticoagulant activity. 19 Plasma, was separated by centrifugation for 15 min at 2000 g at 4 C and immediately stored at À80 C until use.…”

Section: Binding Studies With Surface Plasmon Resonance (Spr)mentioning

confidence: 99%

Pharmacological inhibition of mannose-binding lectin ameliorates neurobehavioral dysfunction following experimental traumatic brain injury

Blasio

Fumagalli

Longhi

et al. 2016

J Cereb Blood Flow Metab

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“…Zipursky and co-workers (40) recently suggested that the anticoagulant properties of another cationic substance, Polybrene, might result from its ability to neutralize the negatively charged phospholipids. Fur-thermore, the report that platelets bear a negative surface charge (41), and the suggestion by Marcus (42,43) that the activity of platelets in clotting may depend upon the platelet membrane, are compatible with this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

The Anticoagulant Activity of Lysosomal Cationic Proteins from Polymorphonuclear Leukocytes *

Saba¹,

Roberts²,

Herion³

1967

J. Clin. Invest.

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Summary. A cationic protein fraction from rabbit polymorphonuclear leukocyte lysosomes has been shown to exert a potent anticoagulant effect on human blood in vitro. The anticoagulant activity is detectable in the whole blood clotting time, the recalcification time of platelet-rich plasma, the prothrombin time, the partial thromboplastin time, and the thromboplastin generation test. The lysosomal cationic proteins do not inhibit any of the known specific procoagulants. They appear to inhibit clotting by blocking the formation of intrinsic thromboplastin possibly by interfering with the role of phospholipids in the reaction involving Factors V and X and calcium.

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