The aim of the study was to test the influence of the mineral salt composition of the medium on in vitro multiplication and growth of kohleria shoots. Shoot tips were cultured on Murashige and Skoog (MS) (full- and half strength), Gamborg et al. (B5), Nitsch and Nitsch (NN) or Lloyd and McCown (WPM) media supplemented with BA 1 mg·dm-3. The influence of passage time on the induction and growth of shoots on the medium containing half strength of MS mineral salts and BA 1 mg·dm-3, GA3 1 mg·dm-3 was also studied. The explants used in the experiments were obtained from aseptically grown shoot clusters. A significant influence of the medium type and the time of propagation on the number, length and weight of axillary shoots was observed. The medium containing full or half strength of mineral salts according to MS was the best. The propagation of kohleria shoots should take place at 4-week passages
The effects of different substrates on flowering of kohleria (Kohleria amabilis Hook.)
S u m m a r yThe aim of the study was to test the effects of different substrates on flowering of Kohleria amabilis Hook. belonging to Gesneriaceae family. Substrates used were: white peat, white peat +bark (1:1), white peat +perlite (1:1), white peat + sand (1:1), coir, low-bog peat, low-bog peat + bark (1:1). Substrates caused effects on flowering of a kohleria. The best of them occured low-bog peat + bark (1:1). Plants bloomed the earliest, for the longest time and plentyfully. Moreover they were hight and nicely leafy. The least useful was white peat.
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