The influence of the mineral composition of the medium on in vitro propagation of Kohleria amabilis (Planch. et Linden) Fritsch shoots

Kozak, D.; Hetman, J.; Witek, M

doi:10.5586/aa.2007.011

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…In addition, juvenile leaves of Sweet Charlie reached the highest number of shoots per explant (4 shoots /explant) compared with other cultivars under analysis. These results are in consensus with previous reports, 38,39 indicating that chemical composition of the media is one of the factors determining the success of in vitro. In addition, selection of the proper hormones is the key to regeneration success in strawberries.…”

Section: Adventitious Shoots Formationsupporting

confidence: 93%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome, which makes the breeding of new cultivars difficult. simple and efficient methods for transformation and regeneration are required for cultivars improvement in strawberries. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., Festival, sweet charlie, and Florida via direct organogenesis using the in vitro juvenile leaves as explants. explants were collected after sub-culturing on a propagation medium composed of Ms supplemented with 0.5 mg/l Ba; 0.1 mg/l Ga3 and 0.1 mg/l IBa. To select the suitable organogenesis, the explants of the three cultivars were cultured on Ms medium supplemented with different concentrations of TDZ (1, 2, 3, and 4 mg/l), then incubated at a temperature of 22 °c ± 2. Medium containing 2 mg/l TDZ revealed the best regeneration efficiency with the three cultivars (72% for Festival and 73% for sweet charlie and Florida). after 4 weeks the produced shoots were cultured on Ms medium with different concentrations of Ba and Kin to enhance shoot elongation. Results showed that the medium containing 1.5 mg/l Ba and 0.5 mg/l Kin revealed highest elongation efficiency (88% and 94%) for Festival and sweet charlie, respectively. On the other hand, medium media containing 1.5 mg/l Ba and 0.1 mg/l Kin showed highest elongation efficiency (90%) in Florida. elongated shoots were successfully rooted on Ms medium containing 1.5 mg/l Naa. Furthermore, transformation of the two cultivars, Festival and sweet charlie, has been established via Agrobacterium strain LBa44404 containing the plasmid pIsV2678 with gus-intron and bar genes. Three days post-cocultivation, GUs activity was screened using the histochemical assay. The results showed 16% and 18% of the tested plant materials had changed into a blue color for Festival and sweet charlie, respectively. Out of 120 explants only 13 shoots were developed on bialaphos medium for each cultivar, representing 10.8% bialaphos-resistant strawberry shoot. The presence of both the genes bar and uid a was detected by PcR and Northern, giving a transformation efficiency of 5%.

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Section: Adventitious Shoots Formationsupporting

confidence: 93%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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“…Among the members of Gesnericaeae, efficient regeneration protocols have been established in Achimenes (Vlahos et al 1995), Saintpaulia (Lo 1997), Didymocarpus (Krishnarajah et al 2002;Shaib et al 2004), Kholeria (Kozak et al 2007), Chirita (Tan et al 2009), Aeschynanthus (Cui et al 2009), Ramonda (Dontcheva et al 2009), Streptocarpus (Wei et al 2010), Metabriggsia (Ma et al 2011), Titanotrichum (Takagi et al 2011), Sinningia (Park et al 2012), Primulina (Qin et al 2013) and Lysionotus (Li et al 2013). The efficient micropropagation system calls for the genetic stability of in vitro regenerated plantlets.…”

Section: Introductionmentioning

confidence: 98%

Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India

Prameela¹,

Ramakrishnaiah²,

Krishna

et al. 2015

Physiol Mol Biol Plants

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Henckelia incana is an endemic medicinal plant used for the treatment of fever and skin allergy. In the present study shoot regeneration was evaluated on Murashige and Skoog's (MS) medium supplemented with auxins, Indole-3-acetic acid (IAA), Indole-3- butyric acid (IBA), 1-Naphthaleneacetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and cytokinins, 6-Benzylaminopurine (BAP) and Kinetin (Kn) at concentrations of 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 mgl(-1). MS medium with IBA (18.08), NAA (17.83) and IAA (17.58) at 0.5 mgl(-1) concentrations showed efficient regeneration. Regenerated shoots were rooted on half-strength MS medium with and without 0.5 mgl(-1) IBA or NAA. The plantlets were successfully hardened in rooting trays (peat, vermiculite and sand) and transferred to field mileu. The genetic fidelity of in vitro raised plants was assessed by using three different single primer amplification reaction (SPAR) markers namely random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and direct amplification of mini-satellite DNA region (DAMD). The results consistently demonstrated true-to-true type propagation. This is the first report of in vitro propagation and establishment of true-to-true type genetic fidelity in H. incana.

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Influence of media and growth regulators on regeneration and morphological characteristics of strawberry cvs Kurdistan and Merck

Mozafari¹,

Gerdakaneh²

2012

Int. J. Plant Physiol. Biochem.

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The influence of the mineral composition of the medium on in vitro propagation of Kohleria amabilis (Planch. et Linden) Fritsch shoots

Cited by 3 publications

References 6 publications

Improved regeneration and transformation protocols for three strawberry cultivars

Improved regeneration and transformation protocols for three strawberry cultivars

Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India

Influence of media and growth regulators on regeneration and morphological characteristics of strawberry cvs Kurdistan and Merck

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