Summary: The occurrence and characteristics of macromolecular components of normal human myometrium and leiomyoma which bind [ 3 H]estradiol and [ 3 H]progesterone were investigated, employing dextran coated charcoal, density gradient centrifugation and gel filtration techniques.On sucrose density gradient centrigugation, [ 3 H]progesterone was bound by macromolecules with sedimentation rates of about 4 S and 8 S.The major [ 3 H]progesterone binding component had a sedimentation coefficient of about 4 S, which contained specific and nonspecific binding sites.Sedimentation patterns as well as elution profiles from agarose gel revealed a striking similarity between biochemical properties of the progesterone receptors from normal myometrium and leiomyomas of the same organ.Both progesterone and estradiql receptor change in concentration during the normal menstrual cycle. During the early proliferative phase the number of estradiol receptor binding sites was highest; after ovulation, a rapid decrease of estradiol receptor level was seen. On the other hand, using [ 3 H]progesterone as the ligand, the highest receptor concentration was found at midcycle.The leiomyoma steroid hormone receptor levels were compared with those in normal myometrium. Whereas leiomyoma exhibited higher estradiol binding capacity, the concentration of progesterone receptors was low in fibroid tumors. In normalem Gewebe variieren die Prqgesterqn-sowie Östradiol-Rezeptorkonzentrationen in der Cytoplasmafraktion. Die höchsten Östradiol-Rezeptorbindungsaktivitäten wurden während der frühen Proliferationsphase beobachtet; nach Oviüation kommt es zu einem rapiden Abfall der Östradipl-Rezeptorkohzentration. Die höchsten ProgesteronRezeptorkonzentratipnen zeichneten sich ab in der Mitte des Menstruationszyklus zum Zeitpunkt der Ovulation. Myome waren charakterisiert durch hohe Östradiql-Bindungskäpazität und eine niedrigere Progesteron-Rezeptorkonzentration. Östrogen-und Progesterön-Rezeptören in normalem Human-Myometrium und Myomen
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.