Lutein is a major carotenoid in soybean [Glycine max (L.) Merr.] seed, and has been shown to be beneficial for eye health in humans. Development of soybeans high in lutein is a goal of some breeding programmes. Little is known about how different growing environments affect lutein content. The objective of this study was to determine the variation of lutein and its relationship to seed protein, oil and individual fatty acids in soybean seed. Fifteen soybean genotypes were planted at four environments. There was no effect of year and planting date on lutein content in soybean seed. However, genotype · year, genotype · planting date and genotype · year · planting date were significant for lutein content. Although each genotype showed similar response across environments, lutein content varied significantly across the four growing environments in 14 of the 15 genotypes evaluated. Lutein content was not correlated with seed protein or oil and palmitic or stearic acid concentrations. However, lutein was positively correlated with oleic acid content and negatively correlated with linoleic and linolenic acids content.

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