The identification of Melaleuca cajuputi leaf samples (trees) that demonstrate enhanced oil characteristics using near infrared (NIR) spectroscopy is described. Leaf samples from an unthinned M. cajuputi seedling seed orchard in Indonesia were collected and air-dried, and their 1,8-cineole content and oil concentrations were determined. NIR spectra of the leaves were obtained, and calibrations for 1,8-cineole content and oil concentration were developed using spectra that had been selected using spectral features; that is, no knowledge of 1,8-cineole content or oil concentration was used to select the calibration samples. The calibrations were used to predict the 1,8-cineole content and oil concentration of the remaining samples. It was demonstrated that NIR spectroscopy could be used to identify leaf samples that had high 1,8-cineole contents and oil concentrations. The technique has the potential to greatly reduce the time involved in ranking large numbers of samples for these attributes, as is a requirement in tree breeding programs to enhance oil production.
Application. Yields of 1,8-cineole and total monoterpenes (as a fraction of leaf weight) from the leaves of 3.75-year-old open-pollinated progeny of Petford E. camaldulensis were highly heritable. Through individual tree selection, substantial improvements in 1,8-cineole yield, the essential oil trait of most commercial interest, and growth rates can be achieved concurrently thus enhancing the economics of growing Petford E. camaldulensis for wood and oil.Abstract. Genetic parameters were estimated for yield of 1,8-cineole and total monoterpenes as a fraction of leaf weight in nineteen open-pollinated families of Petford origin at 3.75 years in two progeny/provenance trials of E. camaldulensis in Zimbabwe. Both traits appear to be highly heritable and, as expected, were strongly genetically correlated, with narrow-sense individual heritabilities near 0.50. Expected gain in the first generation following individual selection in the trials of one tree in 10 for either trait is about 25--32%.Genetic correlations between growth traits and 1,8-cineole yield were small. This indicates that both traits might be improved concurrently thus enhancing the economics of growing Petford E. camaldulensis for wood and medicinal oil. However, the presence of moderate and unfavourable genetic correlations between growth traits and total yield of monoterpenes warrants further study. It was not possible to gauge the significance of apparent family × site interaction for 1,8-cineole yield in this study. However, a test of rank correlation showed an association (Ip < 0.01) between family rankings on each site and the highest-yielding trees on both sites came from the same families. This raises the possibility of being able to select individuals for cloning that yield well over a range of site conditions.
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