In 3 of 15 consecutive patients receiving a human leukocyte antigen (HLA)-identical but major ABO incompatible bone marrow transplant (BMT), pure red cell aplasia (PRA) lasting 5 to 8 months was observed. Titers of the incompatible anti-A agglutinin before infusion of the red blood cell (RBC)-depleted BMT was very high in one, and in the usual range in two patients. Decrease of agglutinin titers during the first 4 weeks after BMT were comparable between PRA patients and those of ABO- incompatible BMT recipients with timely RBC recovery. However, in PRA patients, agglutinin titers rose again and remained elevated for 19 to 28 weeks. RBC engraftment and reticulocyte recovery ultimately occurred spontaneously and coincided with the decrease of agglutinin titers below 16. These observations indicate that PRA is antibody-dependent in this setting. Furthermore, it is conceivable that cyclosporine facilitates recipient-derived antibody synthesis after major ABO- incompatible BMT.
Effective granulocyte transfusion (GT) therapy has been hampered by the low yield of neutrophil granulocytes (PMN) obtainable from normal donors even by use of corticosteroid prestimulation, hydroxyethyl starch (HES), and modern leukapheresis (LA) techniques. To increase the PMN yield we performed LA in 22 healthy volunteer donors after a single subcutaneous administration of 300 micrograms of granulocyte colony- stimulating factor (G-CSF) 12 to 16 hours before LA. Five to 7 L of blood was processed within 1.9 to 3 hours using the standard CS- 3000Plus (Baxter, Deerfield, IL) LA protocol including HES. The mean number of PMN harvested was 44.32 +/- 15.5 x 10(9), corresponding to 6.88 +/- 2.1 x 10(9)/L of blood processed. In the final product PMN functions (in vitro: chemotaxis, phagocytosis, chemiluminescence, superoxide anion production; in vivo: chemiluminescence, half-life) were at least normal. In all donors G-CSF induced a consistent increase of white blood cell (mean 16.46 +/- 3.8 x 10(9)/L) and PMN counts (15.94 +/- 3.6 x 10(9)/L). No G-CSF-related side effects were observed and LA was well tolerated. G-CSF prestimulation allows to harvest three to five times higher numbers of functionally normal PMN by LA compared with corticosteroid pretreatment. This may help to overcome one of the major limitations of an effective PMN support.
In 3 of 15 consecutive patients receiving a human leukocyte antigen (HLA)-identical but major ABO incompatible bone marrow transplant (BMT), pure red cell aplasia (PRA) lasting 5 to 8 months was observed. Titers of the incompatible anti-A agglutinin before infusion of the red blood cell (RBC)-depleted BMT was very high in one, and in the usual range in two patients. Decrease of agglutinin titers during the first 4 weeks after BMT were comparable between PRA patients and those of ABO- incompatible BMT recipients with timely RBC recovery. However, in PRA patients, agglutinin titers rose again and remained elevated for 19 to 28 weeks. RBC engraftment and reticulocyte recovery ultimately occurred spontaneously and coincided with the decrease of agglutinin titers below 16. These observations indicate that PRA is antibody-dependent in this setting. Furthermore, it is conceivable that cyclosporine facilitates recipient-derived antibody synthesis after major ABO- incompatible BMT.
Optical properties are required for the correct understanding and modelling of protoplanetary and debris discs. By assuming that comets are the most pristine bodies in the solar system, our goal is to derive optical constants of real protoplanetary material. We determine the complex index of refraction of the near-surface material of comet 67P/Churyumov-Gerasimenko by fitting the sub-millimetre/millimetre observations of the thermal emission of the comet’s sub-surface made by the Microwave Instrument for the Rosetta Orbiter (MIRO) with synthetic temperatures derived from a thermophysical model and radiative-transfer models. According to the two major formation scenarios of comets, we model the sub-surface layers to consist of pebbles as well as of homogeneously packed dust grains. In the case of a homogeneous dusty surface material, we find a solution for the length-absorption coefficient of α ≈ 0.22 cm−1 for a wavelength of 1.594 mm and α ≥ 3.84 cm−1 for a wavelength of 0.533 mm and a constant thermal conductivity of 0.006 Wm−1K−1. For the pebble scenario, we find for the pebbles and a wavelength of 1.594 mm a complex refractive index of n = (1.074 − 1.256) + i (2.580 − 7.431) · 10−3 for pebble radii between 1 mm and 6 mm. Taking into account other constraints, our results point towards a pebble makeup of the cometary sub-surface with pebble radii between 3 mm and 6 mm. The derived real part of the refractive index is used to constrain the composition of the pebbles and their volume filling factor. The optical and physical properties are discussed in the context of protoplanetary and debris disc observations.
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