Fusarium spp. have recently emerged as significant human pathogens. Identification of these species is important, both for epidemiological purposes and for patient management, but conventional identification based on morphological traits is hindered by major phenotypic polymorphism. In this study, 62 strains, or isolates, belonging to nine Fusarium species were subjected to both molecular identification TEF1 gene sequencing and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) analysis. Following stringent standardization, the proteomic-based method appeared to be both reproducible and robust. Mass spectral analysis by comparison with a database, built in this study, of the most frequently isolated species, including Fusarium solani, Fusarium oxysporum, Fusarium verticilloides, Fusarium proliferatum and Fusarium dimerum, correctly identified 57 strains. As expected, the four species (i.e. Fusarium chlamydosporum, Fusarium equiseti, Fusarium polyphialidicum, Fusarium sacchari) not represented in the database were not identified. Results from mass spectrometry and molecular identification agreed in five of the six cases in which results from morphological and molecular identification were not in agreement. MALDI-TOF yielded results within 1 h, making it a valuable tool for identifying clinical Fusarium isolates at the species level. Uncommon species must now be added to the database. MALDI-TOF may also prove useful for identifying other clinically important moulds.
Context. Comets are primitive objects, remnants of the volatile-rich planetesimals from which the solar system condensed. Knowing their structure and composition is thus crucial for the understanding of our origins. After the successful landing of Philae on the nucleus of 67P/Churyumov-Gerasimenko in November 2014, for the first time, the Rosetta mission provided the opportunity to measure the low frequency electrical properties of a cometary mantle with the permittivity probe SESAME-PP (Surface Electric Sounding and Acoustic Monitoring Experiment−Permittivity Probe). Aims. In this paper, we conduct an in-depth analysis of the data from active measurements collected by SESAME-PP at Abydos, which is the final landing site of Philae, to constrain the porosity and, to a lesser extent, the composition of the surface material down to a depth of about 1 m. Methods. SESAME-PP observations on the surface are then analyzed by comparison with data acquired during the descent toward the nucleus and with numerical simulations that explore different possible attitudes and environments of Philae at Abydos using a method called the Capacity-Influence Matrix Method. Results. Reasonably assuming that the two receiving electrode channels have not drifted with respect to each other during the ten-year journey of the Rosetta probe to the comet, we constrain the dielectric constant of the first meter below the surface at Abydos to be >2.45 ± 0.20, which is consistent with a porosity <50% if the dust phase is analogous to carbonaceous chondrites and <75% in the case of less primitive ordinary chondrites. This indicates that the near surface of the nucleus of 67P/Churyumov-Gerasimenko is more compacted than its interior and suggests that it could consist of a sintered dust-ice layer.
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