Sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and fluorography of in vivo radiolabeled proteins has revealed that protein synthesis in crowns of ‘Midiron’ and ‘Tifgreen’ bermudagrasses (Cynodon dactylon L. Pers. × C. transvaalensis Burtt‐Davy) is altered in association with cold acclimation (CA). Increased synthesis of some proteins in association with CA in Midiron crowns correlates with the greater freezing tolerance of Midiron compared with Tifgreen. This study was undertaken to (i) characterize further alterations in protein synthesis in Midiron and Tifgreen crowns utilizing analytical isoelectric focusing (IEF)/SDS‐PAGE [two‐dimensional (2D‐) PAGE], and (ii) identify cold‐regulated (COR) proteins, proteins encoded by genes persistently upregulated during CA, through sequence analysis of peptides recovered after micropreparative 2D‐PAGE and protease digestion. Plants were grown for 26 d in controlled‐environment chambers under acclimating [8/2°C (day/night) cycles with 10 h photoperiods] or nonacclimating [28/24°C] conditions. Proteins synthesized by isolated crowns were radiolabeled in vivo for 16 h with 35S‐methionine and 35S‐cysteine. Among several groups of COR proteins synthesized in Midiron and Tifgreen crowns, low molecular weight (about 20–28 kilodalton) basic (LB) COR proteins were synthesized in greater amounts and numbers in Midiron than Tifgreen crowns. Among the LB COR proteins, a 27‐kD basic COR protein was synthesized prominently in crowns of both Midiron and Tifgreen plants. This protein from Midiron crowns was designated COR27 and identified as a chitinase (E.C. 3.2.1.14) through protein sequencing.

Neurocysticercosis (NCC) is a parasitic infection of the central nervous system caused by Taenia solium larval cysts. Its epidemiology in cysticercosis-nonendemic regions is poorly understood, and the role of public health institutions is unclear. To determine the incidence of NCC and to pilot screening of household contacts for tapeworms, we conducted population-based active surveillance in Oregon. We screened for T. solium infection by examining hospital billing codes and medical charts for NCC diagnosed during January 1, 2006–December 31, 2009 and collecting fecal and blood samples from household contacts of recent case-patients. We identified 87 case-patients, for an annual incidence of 0.5 cases per 100,000 general population and 5.8 cases per 100,000 Hispanics. In 22 households, we confirmed 2 additional NCC case-patients but no current adult intestinal tapeworm infections. NCC is of clinical and public health concern in Oregon, particularly among Hispanics. Public health intervention should focus on family members because household investigations can identify additional case-patients.

The concentrations of common trace gaseous constituents of the atmosphere have been measured in clean air in rural Illinois and Missouri. Sulfur dioxide (S02), nitrogen dioxide (NO2), ammonia (NH3), and aldehydes (RCHO) were removed from the air by 1 hour of wet scrubbing. Hydrogen sulfide (I-I2S) was collected by passing air through an impregnated filter for I or 2 hours. Background concentrations of SO•, NO2, and RCHO ranged from less than I to 5 ppb. The NI-L concentrations were found to be as high as 10 ppb. The H._,S concentrations varied from less than 0.10 to 0.35 ppb. Comparison with previous measurements and with assumed worldwide values indicates that the concentrations of NO2 and I-LS assumed

Neurocysticercosis (NCC) is a parasitic infection of the central nervous system caused by Taenia solium larval cysts. Its epidemiology in cysticercosis-nonendemic regions is poorly understood, and the role of public health institutions is unclear. To determine the incidence of NCC and to pilot screening of household contacts for tapeworms, we conducted population-based active surveillance in Oregon. We screened for T. solium infection by examining hospital billing codes and medical charts for NCC diagnosed during January 1, 2006–December 31, 2009 and collecting fecal and blood samples from household contacts of recent case-patients. We identified 87 case-patients, for an annual incidence of 0.5 cases per 100,000 general population and 5.8 cases per 100,000 Hispanics. In 22 households, we confirmed 2 additional NCC case-patients but no current adult intestinal tapeworm infections. NCC is of clinical and public health concern in Oregon, particularly among Hispanics. Public health intervention should focus on family members because household investigations can identify additional case-patients.

Ten electrical moisture meters were tested with 159 samples of Canadian hard red spring wheat, of Grades 1, 2, 3, and 4 Northern, representing a moisture range of 11 to 17%. The two-stage vacuum oven and Brown–Duvel methods were used as bases of comparison. Regression equations, standard errors of estimate, and data on temperature effects are reported. The standard error of estimate of vacuum oven results was 0.15% for the Brown–Duvel method, 0.23% for the Tag–Heppenstall meter, and 0.28% for the Universal meter. Other meters had higher errors of estimate.

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The objective of this study was to evaluate a recombinant inbred line population derived from a cross between a recombinant inbred line (RIL) resistant to sudden death syndrome (SDS). 'LS90-1920' with a susceptible line, 'Spencer' in order to identify any significant association between yield and important agronomic traits with SDS, estimate heritability of these traits and determine whether there are traits that can be used as predictors for SDS resistance. Correlation coefficients for yield and agronomic traits (maturity, lodging, and plant height) were moderately to highly significant but there was no significant association between these traits and SDS resistance. Genotype by environment interaction was significant for all traits studied except of plant height. Maturity, lodging, plant height and SDS resistance were moderately to highly heritable whereas yield showed very low heritability. Our findings showed that environment plays a very crucial role in selection. It is showed that genotypic selection can speed up but cannot replace phenotypic selection across environments and time. Environment is important for the development and production of crop plants because it optimizes the association between the genotype and the phenotype. Highlights: Created Recombinant Inbred Line; Tested for agronomic traits including yield; Tested for disease resistance; Analyzed results to determine if Recombinant Inbred Line differed from the parental lines; Determined if traits were inherited from parents.