The aim of the study was to evaluate the anti-cariogenic effects of Lactobacillus salivarius by reducing pathogenic species and biofilm mass in a double-species biofilm model. Coexistence of S. mutans with C. albicans can cause dental caries progression or recurrence of the disease in the future. Fifty-nine children with diagnosed early childhood caries (ECC) were recruited onto the study. The condition of the children’s dentition was defined according to the World Health Organization guidelines. The participants were divided into children with initial enamel demineralization and children showing dentin damage. The study was performed on the S. mutans and C. albicans clinical strains, isolated from dental plaque of patients with ECC. The effect of a probiotic containing Lactobacillus salivarius on the ability of S. mutans and C. albicans to produce a double-species biofilm was investigated in an in vitro model. The biomass of the formed/non-degraded biofilm was analyzed on the basis of its crystal violet staining. The number of colonies of S. mutans and C. albicans (CFU/mL, colony forming units/mL) forming the biofilm was determined. Microorganism morphology in the biofilm was evaluated using a scanning electron microscope (SEM). In vitro analysis demonstrated that the presence of S. mutans increased the number of C. albicans colonies (CFU/mL); the double-species biofilm mass and hyphal forms produced in it by the yeast. L. salivarius inhibited the cariogenic biofilm formation of C. albicans and S. mutans. Under the influence of the probiotic; the biofilm mass and the number of S. mutans; C. albicans and S. mutans with C. albicans colonies in the biofilm was decreased. Moreover; it can be noted that after the addition of the probiotic; fungi did not form hyphae or germ tubes of pathogenic potential. These results suggest that L. salivarius can secrete intermediates capable of inhibiting the formation of cariogenic S. mutans and C. albicans biofilm; and may inhibit fungal morphological transformation and thereby reduce the pathogenicity of C. albicans; weakening its pathogenic potential. Further research is required to prove or disprove the long-term effects of the preparation and to achieve preventive methods.
The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children. The study group included of 63 children aged 2–6 years: 32 with caries and 31 without caries. The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II). The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste. The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children. The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test). A similar relationship was also observed for frequent snacking between meals (p = 0.0038, chi-square test). The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries. Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11–33.44). The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
Secondary caries is a disease associated with the formation of biofilm on the border of the tooth and dental filling. Its development is strongly influenced by the dietary sweet foods and the type of dental material. The aim of the study was to assess the effect of sweeteners on the ability of clinical Streptococcus mutans strains to form biofilm on dental materials. Strains were isolated from plaque samples from 40 pediatric patients from the 3–6 ICADS II group. The ability to form biofilm was tested on composite and glass ionomer dental materials used for milk teeth filling in the presence of sucrose, xylitol, sorbitol, and erythritol. The bacterial film mass after 12, 24, 48, and 72 h and the number of bacterial colonies significantly decreased (p < 0.01) compared to the initial value for 5% erythritol and sorbitol on examined materials. A greater inhibitory effect was noted for glass ionomers compared to composites. Sucrose and xylitol supported biofilm formation, while erythritol had the best inhibitory effect. The use of fluoride-releasing glass ionomers exerted an effect synergistic to erythritol, i.e., inhibited plaque formation and the amount of cariogenic S. mutans. Selection of proper type of dental material together with replacing sucrose with polyols can significantly decrease risk of secondary caries development. Erithritol in combination with glass ionomer seems to be the most effective in secondary caries prevention.
Introduction. In 2016, 5-, 7- and 12-year-old children from the Małopolskie region were included in the study of oral health as part of the Nationwide Monitoring of Oral Health programme, which has been periodically implemented in selected index groups of the Polish population since 1997. Aim. To evaluate the prevalence and level of dental caries in 5-, 7- and 12-year-old children from the Małopolskie region and to observe the dental caries trend in this region in recent years in relation to the Polish population. Material and methods. The study covered 673 children from the Małopolskie region, including 223 aged 5, 225 aged 7 and 225 aged 12 years. Dental condition/state of dentition was examined according to the WHO recommendations and criteria. The prevalence and level of dental caries in children from the Małopolskie region were evaluated and compared with the results of earlier studies from this province. The obtained results were also compared with the results for the entire Polish population from the current and previous nationwide monitoring studies. The studies were conducted upon the consent of the Medical University of Warsaw Ethics Committee no. KB 190/2016. Results. Dental caries prevalence in 5-, 7- and 12-year-old children from the Małopolskie region that were covered by the study was 74.9, 84.9 and 81.8%, respectively. The average values of dmft were 4.55 (in the age group of 5) and 5.38 (in the age group of 7), while DMFT for particular age groups was 0.03, 0.56 and 3.54, respectively. Slight decrease in the value of these indications of caries is noticed for 5- and 7-year-old children from the Małopolskie region (decrease in prevalence by 2.5 and 6.7%, respectively, as well as a fall in the value of dmft by 0.71 in the age group of 5, relative to 2011). The group of 12-year-old children from our region, as the only one from the entire Polish population of children of this age, have kept a downward trend of caries prevalence (a fall by 12.7% relative to the monitoring study from 2010). This reduction needs to be confirmed in the next monitoring studies. Conclusions. The presented results indicate stable high prevalence of dental caries in children of the Małopolskie region and in the whole country. It is therefore necessary to enhance actions intended to improve this situation through education of both children and their parents, widespread prophylaxis, and greater health-oriented awareness of patients.
Objective: Dental caries is the most prevalent chronic disease in childhood. It is a social disease, which causes search for effective methods of its prevention. Due to its inhibitory action on Streptococcus mutans, xylitol is a readily used supplement. The purpose of the study was a determination of the effect of 0.01% concentration of xylitol on monospecies S. mutans biofilm formation.Design: Clinical strains were acquired from 64 children aged 4.7 ± 0.81 years. The study group included children with caries and patients without carious lesions. The isolates identified using a MALDI-TOF mass spectrometer. The effect of 0.01% xylitol on monospecies biofilm formation after 8, 24, 48 and 72 h of culturing was evaluated. The produced biofilms were evaluated by determining the biofilm biomass, microorganism concentration and analyzing structure in a scanning electron microscope. Results:The biofilm biomass treated with xylitol was, at every time point, significantly lower (p < 0.001), in both study groups of children compared to the biofilm not treated with xylitol. A statistically significant decrease in the biofilm-forming capacity with xylitol in CFU/mL was obtained after 48 h in both groups. Conclusions:The anti-biofilm efficacy of xylitol at low concentrations, justifies its use in caries prevention in children.
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