BackgroundZika virus (ZIKV) is an emergent threat provoking a worldwide explosive outbreak. Since January 2015, 41 countries reported autochthonous cases. In Brazil, an increase in Guillain-Barré syndrome and microcephaly cases was linked to ZIKV infections. A recent report describing low experimental transmission efficiency of its main putative vector, Ae. aegypti, in conjunction with apparent sexual transmission notifications, prompted the investigation of other potential sources of viral dissemination. Urine and saliva have been previously established as useful tools in ZIKV diagnosis. Here, we described the presence and isolation of infectious ZIKV particles from saliva and urine of acute phase patients in the Rio de Janeiro state, Brazil.Methodology/Principal FindingsNine urine and five saliva samples from nine patients from Rio de Janeiro presenting rash and other typical Zika acute phase symptoms were inoculated in Vero cell culture and submitted to specific ZIKV RNA detection and quantification through, respectively, NAT-Zika, RT-PCR and RT-qPCR. Two ZIKV isolates were achieved, one from urine and one from saliva specimens. ZIKV nucleic acid was identified by all methods in four patients. Whenever both urine and saliva samples were available from the same patient, urine viral loads were higher, corroborating the general sense that it is a better source for ZIKV molecular diagnostic. In spite of this, from the two isolated strains, each from one patient, only one derived from urine, suggesting that other factors, like the acidic nature of this fluid, might interfere with virion infectivity. The complete genome of both ZIKV isolates was obtained. Phylogenetic analysis revealed similarity with strains previously isolated during the South America outbreak.Conclusions/SignificanceThe detection of infectious ZIKV particles in urine and saliva of patients during the acute phase may represent a critical factor in the spread of virus. The epidemiological relevance of this finding, regarding the contribution of alternative non-vectorial ZIKV transmission routes, needs further investigation.
Zika virus (ZIKV) has caused a major epidemic in Brazil and several other American countries. ZIKV is an arbovirus whose natural vectors during epidemics have been poorly determined. In this study, 1,683 mosquitoes collected in the vicinity of ZIKV suspected cases in Rio de Janeiro, Brazil, from June 2015 to May 2016 were screened for natural infection by using molecular methods. Three pools of Aedes aegypti were found with the ZIKV genome, one of which had only one male. This finding supports the occurrence of vertical and/or venereal transmission of ZIKV in Ae. aegypti in nature. None of the examined Ae. albopictus and Culex quinquefasciatus was positive. This is the first report of natural infection by ZIKV in mosquitoes in Brazil and other South American countries. So far, Ae. aegypti is the only confirmed vector of ZIKV during the ongoing Pan-American epidemics.
The current outbreak of yellow fever virus (YFV) that is afflicting Brazil since the end of 2016 probably originated from a re-introduction of YFV from endemic areas into the non-endemic Southeastern Brazil. However, the lack of genomic sequences from endemic regions hinders the tracking of YFV’s dissemination routes. We assessed the origin and spread of the ongoing YFV Brazilian outbreak analyzing a new set of YFV strains infecting humans, non-human primates (NHPs) and mosquitoes sampled across five Brazilian states from endemic and non-endemic regions between 2015 and 2018. We found two YFV sub-clade 1E lineages circulating in NHP from Goiás state (GO), resulting from independent viral introductions into the Araguaia tributary river basin: while one strain from 2017 clustered intermingled with Venezuelan YFV strains from 2000, the other YFV strains sampled in 2015 and 2017 clustered with sequences of the current YFV outbreak in the Brazilian Southeastern region (named YFV 2015-2018 lineage), displaying the same molecular signature associated to the current YFV outbreak. After its introduction in GO at around mid-2014, the YFV 2015-2018 lineage followed two paths of dissemination outside GO, originating two major YFV sub-lineages: (1) the YFV MG/ES/RJ sub-lineage spread sequentially from the eastern area of Minas Gerais state to Espírito Santo and then to Rio de Janeiro states, following the Southeast Atlantic basin; (2) the YFV MG/SP sub-lineage spread from the southwestern area of Minas Gerais to the metropolitan region of São Paulo state, following the Paraná basin. These results indicate the ongoing YFV outbreak in Southeastern Brazil originated from a dissemination event from GO almost 2 years before its recognition at the end of 2016. From GO this lineage was introduced in Minas Gerais state at least two times, originating two sub-lineages that followed different routes toward densely populated areas. The spread of YFV outside endemic regions for at least 4 years stresses the imperative importance of the continuous monitoring of YFV to aid decision-making for effective control policies aiming the increase of vaccination coverage to avoid the YFV transmission in densely populated urban centers.
Objetivos: Analizar la presencia de estrés y signos indicativos de depresión en docentes de una institución pública de enseñanza.Material y Métodos: Estudio analítico, realizado con 163 docentes de una institución pública de enseñanza. Se aplicó Escala de estrés percibido-EEP e inventario de depresión de Beck-IDB.Resultados: De los 163 participantes, 108 (66,3%) eran del género masculino, 64 (39,3%) con rango de edad de 30 a 39 años, 79 (48,5%) tenían una renta salarial de más de 15 salarios (en el caso de las mujeres). La mayoría presentó título de maestro, 84 (51,5%). Se identificaron niveles mínimos de estrés en hombres, 73 (67,0%) y 34 (63,0%) en mujeres. 87 (79,8%) mujeres y 35 (64,8%) hombres presentaron un nivel de depresión mínima. Hubo diferencias significativas en los escores de estrés y depresión entre los sexos. Se observó una correlación positiva moderada entre las escalas.Conclusión: Los docentes con niveles mínimo y leve están sujetos a presentar estrés y depresión, mereciendo atención por parte de las instituciones. Objectives: To analyze the presence of stress and signs indicative of depression in teachers of a public educational institution.Materials and Methods: An analytical study was conducted with 163 teachers from a public educational institution. The Perceived Stress Scale-EEP and Beck-IDB Depression Inventory were applied.Results: Of the 163 participants, 108 (66.3%) were males, 64 (39.3%) were between 30 and 39 years of age, 79 (48.5%) had a wage income of more than 15 wages minimum, 80 (49.1%) reported brown color. The majority had a master's degree, 84 (51.5%). Minimal levels of stress were identified in men, 73 (67.0%) and 34 (63.0%) in women. 87 (79.8%) women and 35 (64.8%) men had minimal depression. There was a significant difference in the stress and depression scores between the sexes. There was a moderate positive correlation between the scales.Conclusion: Teachers with minimal and light levels are subject to stress and depression, deserving attention from the institutions. Objetivos: Analisar a presença de estresse e sinais indicativos de depressão em docentes de uma instituição pública de ensino. Material e Métodos: Estudo analítico, realizado com 163 docentes de uma instituição pública de ensino. Aplicou-se Escala de Estresse Percebido-EEP e Inventário de Depressão de Beck-IDB. Resultados: Dos 163 participantes, 108 (66,3%) eram do gênero masculino, 64 (39,3%) com faixa etária de 30 a 39 anos, 79 (48,5%) tinham uma renda salarial de mais de 15 salários mínimos, 80 (49,1%) referiram cor parda. A maioria apresentou título de mestre, 84 (51,5%). Identificaram-se níveis mínimos de estresse em homens, 73 (67,0%) e 34 (63,0%) em mulheres. 87 (79,8%) mulheres e 35 (64,8%) homens apresentaram nível de depressão mínima. Houve diferença significativa nos escores de estresse e depressão entre os sexos. Observou-se uma correlação positiva moderada entre as escalas. Conclusão: Os docentes com níveis mínimo e leve estão sujeitos a apresentarem estresse e depressão, merecendo atenção por parte das instituições.
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