Chronic pancreatitis and pancreatic adenocarcinoma are extensively studied as common and potentially lethal disorders. However, their causes and genetic background in most cases remain unclear. The C677T polymorphism in 5',10'-methylenetetrahydrofolate reductase (MTHFR) gene may modulate the risk of pancreatic disorders. In this study, we tested whether MTHFR C677T polymorphism is associated with chronic pancreatitis and pancreatic adenocarcinoma in the Serbian population. DNA was extracted from blood samples of 51 chronic pancreatitis patients, 21 pancreatic adenocarcinoma patients, and a control group consisting of 50 healthy smokers. The MTHFR C677T polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Although, no statistically significant differences were observed in the distribution of MTHFR genotype or allele frequencies between patients and control groups, the results showed an increased frequency of homozygotes for MTHFR C677T polymorphism in chronic pancreatitis patients (14%) and a decreased frequency in pancreatic adenocarcinoma patients (5%) in comparison to the control group (8%). We speculate that the MTHFR C677T polymorphism could act as a possible risk factor for chronic pancreatitis and a possible protective factor in pancreatic adenocarcinoma. This observation needs further investigation in prospective studies on a larger number of patients, in which the effect of other genetic and environmental factors should also be taken into consideration.
Kratak sadr`aj: Cilj ove studije bio je da se odredi u~e stalost polimorfizma Ile105Val u genu za glutation S-trans ferazu P1 (GSTP1) i proceni njegov uticaj na rizik za obolevanje od pankreasnih bolesti u srpskoj populaciji. Studija je obuhvatila 157 pacijenata sa naj~e{}im bolestima pankreasa: 47 sa karcinomom pankreasa, 50 sa hroni~nim pankre atitisom i 60 sa dijabetes melitusom tipa 2, kao i 107 zdravih ispitanika. Prisustvo polimorfizma Ile105Val u genu za GSTP1 je analizirano metodom PCR-RFLP (Polymerase Chain Reaction -Restriction Fragment Length Polymorphism). U~estalost alela 105Val je bila ni`a kod pacijenata sa karcinomom pankreasa (24,5%) i hroni~nim pankreatitisom (24,0%) i neznatno povi{ena kod pacijenata sa dijabe tes melitusom tipa 2 (31,7%) u odnosu na u~estalost kod zdra vih ispitanika (29,9%), ali razlike nisu bile statisti~ki zna ~aj ne. U~estalost pojedinih genotipova polimorfizma Ile105Val se razlikovala me|u analiziranim grupama, ali razlike tako|e nisu bile statisti~ki zna~ajne. Samo nekoliko studija se do sada bavilo ulogom GSTP1 Ile105Val poli morfizma u bole stima pankreasa i uglavnom su dale opre ~ne rezultate. Zna ~aj GSTP1 Ile105Val polimorfizma za bolesti pankreasa jo{ uvek nije razja{njen i potrebna su da lja istra`iva nja da bi se ispitala njegova uloga u pankreas nom tkivu.Klju~ne re~i: glutation S-transferaza P1 (GSTP1), polimor fizam Ile105Val, karcinom pankreasa, hroni~ni pankreatitis, dijabetes melitus tipa 2
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