Imbalance in the resident microbiota may promote the growth of opportunistic microorganisms, such as yeasts of Candida genus and the development of diseases, especially in aged people. This study evaluated whether the consumption of the probiotic Yakult LBz(®) (Lactobacillus casei and Bifidobacterium breve) was able to influence on the specific immunological response against Candida and on the presence of these yeasts in the oral cavity of 42 healthy aged individuals. Saliva samples were collected before and after the probiotic use for 30 days, 3 times a week. The samples were plated in Dextrose Saboraud Agar with chloramphenicol, the colony-forming units (CFU/mL) were counted and the Candida species were identified. Anti-Candida IgA analysis was conducted using the ELISA technique. ANOVA and Student's t-test were used for normally distributed data and the Wilcoxon test was used for data with non-normal distribution (α=0.05). The results showed a statistically significant reduction (p<0.05) in Candida prevalence (from 92.9% to 85.7%), in CFU/mL counts of Candida and in the number of non-albicans species after consumption of the probiotic. Immunological analysis demonstrated a significant increase (p<0.05) in anti-Candida IgA levels. In conclusion, probiotic bacteria reduced Candida numbers in the oral cavity of the elderly and increased specific secretory immune response against these yeasts, suggesting its possible use in controlling oral candidosis.

Candida spp. was found in the saliva of 80% of the individuals in the test group and 65% of the control, with C. albicans being the most prevalent species. The test group presented with the highest number of colony forming units of Candida per ml of saliva, and there was weak correlation between this number and the metal and resin area of the denture (Pearson's coefficient of correlation). Greater prevalence and a higher number of colony forming units of Candida per ml of saliva occurred in removable partial denture wearers (p = 0.04) with a weak positive correlation between the metal and resin area and the number of colony forming units of Candida per ml of saliva. However, this correlation was more significant for the area of resin.

The purpose of this study was to determine if citric-acid solutions for endodontic use can become contaminated and if the addition of a microbiological stabilizer to the acid would modify this situation. Five types of microorganisms were inoculated in tubes containing 10% citric-acid solutions, pH 1.8 (group A1), and 10% solution, pH 1.8, combined with 0.1% sodium benzoate (group A2). The results demonstrated that solutions of group A1 were contaminated with Candida albicans in 100% of the tubes, Escherichia coli in 80%, and Enterococcus faecalis in 50%. When stabilizer was added (group A2), C. albicans grew in only 30% of tubes and all bacterial species were neutralized.

Quando se pretende estabelecer o risco de cárie de diferentes populações, inúmeros fatores têm se mostrado importantes, incluindo aspectos clínicos, microbiológicos, condições sócio- econômicas e hábitos dos indivíduos. O objetivo do presente estudo foi avaliar a condição bucal e o risco de cárie dos alunos do 1o ano da FOSJC/UNESP logo após o ingresso dos mesmos (15 dias do início das aulas). Foram examinados 73 adultos jovens (19,66 ± 3,02 anos de idade), nos quais observou-se CPO-D e índice de PASS. Os hábitos de higiene bucal, exposição ao flúor, acesso a cuidados odontológicos bem como a presença de sangramento gengival foram analisados por meio de criteriosa anamnese. A dieta foi avaliada através de preenchimento de diário alimentar. Amostras de saliva foram obtidas de cada indivíduo a fim de avaliar fluxo e capacidade tampão da saliva, produção de ácidos pelos microrganismos bucais, presença e quantificação de estreptococos do grupo mutans, Lactobacillus e Candida. O risco de cárie baseado nos dados microbiológicos foi estabelecido através de escores. Os resultados mostraram alta prevalência de placa bacteriana e sangramento gengival, CPOD médio de 13,86 e baixo risco de cárie nos alunos ingressantes do curso de Odontologia