Butterflies are insects that live cosmopolitan. Some butterflies in tropical forests look for food sources from sucking the juice of ripe fruits that have fallen on the forest floor. Fruit-feeding butterflies can adapt in finding food sources, selecting and sucking food effectively. Fruit contains varying concentrations of sugar and nitrogen. The existence of fruit-feeding butterflies in tropical rain forests is influenced by the availability and quality of food sources as well as other supporting factors such as temperature, humidity, and light intensity. The purpose of this study was to identify fruit-feeding butterflies and their preferences for banana and pineapple baits in the Soraya Research Station Area, Leuser Ecosystem, Aceh, Indonesia from September to November 2020. Six forest trail locations were selected as trap locations. A total of 360 cylindrical gauze baited traps (80 cm high and 35 cm diameter) were installed on six trails/line transects. Baited traps were set at three different heights, that is at the understorey level (± 0-2 m), midstorey level (± 5-6 m), and overstorey level (± 10-11 m) with a distance of ± 5-10 m. The distance between location points is ± 250 m. The fruit baits used in this trap were ripe bananas and pineapples. The results obtained were 37 species with 176 individuals trapped on banana bait and 50 species with 183 individuals on pineapple bait. However, the results of the t-test using the Man-Whitney test showed no significant difference between the banana and pineapple bait used.
BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre- freezing temperatures of 4°C, −10°C, and −79°C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at −179°C for 7 days. RESULTS: The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (ρ<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10% DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.
The principle of sperm preservation is to prolong the viability of spermatozoa by reducing the degree of metabolism during storage at low temperatures, and one method involved was a cryoprotectant as a sperm protector. This study aimed to determine the best type of cryoprotectant for refrigeration storage of goldfish Carrasius auratus sperm. A completely randomized design was used, and five types of cryoprotectant were tested, namely Methanol, Ethanol, Ethylene Glycol, Glycerol, and DMSO at a 10% concentration combined with 15% egg yolks. The sperm was diluted with Ringer’s solution and stored at 4⁰C for 48 hours. The ANOVA test revealed that cryoprotectant significantly affected the motility and viability of sperm after 48 hours of refrigeration (P0.05). The results showed that 10% DMSO combined with 15% egg yolk could maintain sperm motility and viability better than other treatments, with values of 85.80% and 86.50%, respectively, which were significantly different from the other treatments. Glycerol produced the second-best sperm quality with motility and viability values of 71.13% and 74.13%, respectively. Therefore, DMSO is the most effective cryoprotectant for refrigeration storage of goldfish sperm.
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