Indigenous Trichoderma has widely used in late-blight epidemic areas in Magelang, Indonesia as biocontrol and biomodulator. But it application still lacks of scientific proof, even though it is potently to be developed. Therefore, this study aims to identify and evaluate indigenous Trichoderma application in eradicating late blight and increase productivity. This research was an experimental posttest-only using six experimental plots, with P0 plot with no treatment. The solution was made by dissolve indigenous Trichoderma solid-starter into water (m/v), then used for treatment. The treatment plots including: two groups of sprayed-only plots with doses of 100 g/ 10 L of solution for P1 and 150 g/ 10 L of solution for P2; and two group treated with sprayed-poured method using doses of 100 g/ 10 L of solution for P3 and 150 g/ 10 L of solution for P4. The positive control group was P5 group, treated using chemical pesticides and tuber booster. The microscopic identification revealed that the local bioagent was Trichoderma harzianum species. Application of T. harzianum in P4 was significantly increased the productivity. However, it was not effective in increasing growth, but able to reduce the intensity of late blight disease. The research revealed that native Trichoderma is able to be used as anti-infectious agent and potentially improve the quality of potato plants. In the future, this research may worthwhile for farmers to develop and produce trustworthy and proven Trichoderma-based biocontrol and help them increase the potato production economically.
Alkaline protease is an industrially important enzyme that can produce by Aspergillus flavus DUCC K225 an indigenous mold from lime soil Madura island. The production of alkaline protease by Aspergillus flavus DUCC K225 produced by submerged fermentation on modified Czapeks Dox medium containing rice bran as N source. The enzyme production was observed after 7th-day incubation, by measuring the protease activity at pH 8.5 and the temperature stability as well. The results showed that the enzyme activity is higher on the rice bran medium compared to the standard medium, with a value of 237,84 U/ml and 94.85 U/ml respectively. This alkaline protease enzyme produced also thermostable, with 89.3% stability value for 60 min at 40°C.
Oral health is an important thing that must be prioritized because the entire intake is first processed in the mouth. The most common disease found in the oral cavity is candidiasis, caused by Candida albicans which is an opportunistic intraoral pathogen that inhabits the oral cavity. Peppermint (Mentha piperita L.) is one of the herbal plants which has proven in laboratory scale have antifungal activity. This research aimed to know the antifungal activity of methanolic and chloroform extract of M. Piperita L. leaves against C. albicans. Mint leaf extract was obtained by maceration method using methanol and chloroform as solvents. The extract concentration for antifungal activity test were 40%; 60% and 80% w/v solution in DMSO 100%. The Kirby-Bauer method was used to examine the antifungal activity of both extracts. The results showed that both methanolic and chloroform extract has antifungal activity against C. albicans. The antifungal of methanolic better than the chloroform extract. Both extracts at all concentration showed a greater antifungal activity compared to 25.000 μg ketoconazole as a positive control. The best antifungal activity of methanolic mint leaves extracts found at 80% concentration. It is no different in antifungal activity of chloroform mint leaves extract at all concentration tested.
Mycorrhiza is a root plant symbiont functioning to increase mineral and nutrients absorption. Mycorrhiza fertilization to potatoes has to be monitoredin order to observethe effects of mycorrhiza on the growth of potatoes. Therefore, theaim of thisstudy was to analyze the effect of mycorrhiza on potato growth and production. This field research was conducted in Kledung village, Temanggung (1,138 masl) from 25 March to 25 June 2018. The method used was separated block design with 4 treatments and 20 replications in each plot. The first plot (P1) was the plot without treatment; the second plot (P2), positive control, was the plot treated with chemical fertilizer;the third plot (P3) was plot treated with organic fertilizer Bokasi and Trichoderma; and the fourth plot (P4) was fertilized using mycorrhiza. The data related to growth and production were analyzed using ANOVA and Tukey’s test; while, to determine the best type of fertilizer among treatments t-testwas used. The results showed that mycorrhizal application significantly increased height (20.12 cm p ≤ 0.05), the number of leaf (32.47, p ≤ 0.05), and potato production (213.70 gr). It can be concluded that the use of mycorrhiza significantly increased the growth and production of potato.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.