The objective of the present study was to investigate the effect of eggshell powder supplementation in diet on the growth and survival rate of black tiger shrimp, Penaeus monodon larvae. A total of 600 shrimp larvae (PL 12) was used with five treatment levels and four replications. The shrimp fed with 42% of crude protein, feeding frequency three times a day at 08.00; 14.00; 18.00 pm and 5% feeding rate. The dietary treatments were A (0%), B (3%), C (6%), D (9%) and E (12%) fed for thirty days. Results showed that the supplementation of eggshell powder in diet had a significant effect on weight gain, length gain, specific growth rate, and survival of black tiger shrimp larvae. Duncan's advanced test showed that the optimum dose of eggshell powder was found in treatment A (0% eggshell powder) with weight gain, length gain, specific growth rate, and survival rate at 0.0301±0,0013 g, 12.41±0,56 mm, 12.82±0,16 %/day, and 94.17±1,66%, respectively. Hence, it can be concluded that the supplementation of eggshell powder may decrease the growth and survival rate of black tiger shrimp larvae.