Prosopis farcta is belonged to Mimosaceae family, commonly known as mesquite. It was chosen to investigate their effect on α-glucosidase inhibitory activity (in-vitro), serum glucose and liver functions. Roots, fruits, and leaves of P. farcta, were extracted by n-hexane, ethyl acetate, and methanol. α-glucosidase inhibition was analyzed by using ELISA technique then half maximal inhibitory concentration IC50 was found. The blood glucose levels were determined with a glucose analyzer model. The serum alkaline phosphatase (ALP), Alanine transaminase (ALT), aspartate aminotransferase (AST) and serum total bilirubin (TB) were estimated by using the Cobas diagnostic kit with a fully automated chemical analyzer. Diabetes was done by a single dose of 120 mg alloxan/kg b.w with subcutaneously injection. Ethyl acetate extracts of P. farcta showed the higher α-glucosidase inhibitory activity, and the best one was root extract. Treatments of the alloxan-induced diabetes rats were done by daily oral administration of different concentrations with P. farcta extracts of ethyl acetate for 28 days and the dose 200 mg/kg BW was the effective one. The root extract was the best one for reduction of serum glucose followed by leaves then fruits. Administration of root extract of P. farcta showed a decrease in the levels of ALP and TB in alloxan-induced diabetes rats. The fruit extract of P. farcta showed decreasing in the level of ALP in alloxan-induced diabetes rats. In conclusion, the P. farcta extracts for ethyl acetate have properties of hypoglycemic effect as well as improving some parameters related with diabetic complications of liver functions.

The current investigation aimed to test the alterations of the levels of obestatin hormone, lipid profile and cardiac function markers in relation to hyperglycemia in patients with non-insulin diabetes mellitus. The study included 118 diabetic subjects (56 males, 62 females) and 60 healthy non-diabetic subjects (30 males, 30 females). Diabetic and healthy subjects were age-matched. Serum levels of obestatin, lipid profile markers including total cholesterol (STC), triglycerides (STG), low and high density lipoproteins (LDL-C and HDL-C), as well as cardiac function markers including, creatine kinase and lactate dehydrogenase enzymes were determined in all subjects. The findings revealed a remarkable decrease in the level of serum obestatin in both diabetic males and females with both age ranges (40-59 and 60-80 years). On the other hand, the results showed that serum STC, LDL-C, and STG levels were statistically significantly elevated, while that for HDL-C was significantly decreased in diabetic males and females with both age ranges. Concerning the cardiac markers, the results found out that the levels of CK-MB and LDH were significantly increased in type 2 diabetic males and females with both age ranges. The results suggested that a low concentration of obestatin is a significant risk factor for type 2 DM, with a key role for this hormone in the pathogenesis. Accordingly, altered levels of obestatin could be used as an important indicator for type 2 DM. The current study also suggests a direct relationship between lipid profiles, except for HDL, the decreased obestatin level, the increased cardiac function markers, and hyperglycemic status in type 2 DM.

Thirty male albino rats were used to investigate the effect of green tea extract on pancreas, renal and hepatic histological parameters in alloxan induced diabetes mellitus. The animals were randomly distributed into five groups of six animals in each group. The first group was regarded as normal healthy control, the second group was regarded as diabetic control, the third group was treated with insulin, the fourth group was treated with green tea extract, 50 mg/kg body weight (GTE50), and the fifth group was treated with GTE100. This study showed that functionally diabetes related organs such as pancreas, kidney and liver showed diabetes related pathological changes and these revealed a noticeable tendency for reversion to normal after treatment with green tea extract.

Complications associated with diabetes are a consequence of acute disturbance in glucose metabolism in a human body. The most significant complication of diabetes is bone disorders which contributes to high levels of bone disability. This study included 118 diabetic patients, 56 males, 62 females, and 60 healthy non-diabetic controls, 30 males, 30 females. The patients and controls were age matched. Circulating levels of bone function markers (osteoprotegerin, vitamin D, PTH, total calcium and inorganic phosphorus) were determined in all subject groups. The data obtained from this study showed that the serum levels of osteoprotegerin had significantly increased in both diabetic male & female in both age ranges which were 496.3±61.46 pg/mL & 335.7±29.33 pg/mL; 329.8±48.78 pg/mL & 219.9±18.72 pg/mL respectively, in comparison with its level in control matched age ranges 294.6±26.19 pg/mL & 226.8±28.07 pg/mL; 215.7±31.85 pg/mL & 171.9±14.19 pg/mL respectively. Serum calcium concentration had non-significantly increased both in the diabetic males and females in both age ranges which were 11.10±0.46 mg/dL & 11.76±0.74 mg/dL; 10.33±0.33 mg/dL & 10.28±0.48 mg/dL respectively, when compared with its level in control matched age ranges 10.46±0.34 mg/dL & 10.14±0.35 mg/dL;9.69±0.41 mg/dL & 10.08±0.45 mg/dL. Serum vitamin D, parathyroid hormone (PTH) and inorganic phosphorus concentrations had significantly decreased both in the diabetic male and female subjects in both age ranges which were 5.78±1.30 ng/mL and 2.47±0.12 ng/mL; 9.47±1.98 ng/mL, and 10.70±2.11 ng/mL; 74.78±7.42 pg/mL & 67.83±3.69 pg/mL;42.94±2.00 pg/mL & 15.51±1.98 pg/mL; 4.34±0.27 mg/dL & 4.76±0.35 mg/dL; 4.38±0.21 mg/dL; 5.12±0.44 mg/dL respectively when compared with their level in control matched age ranges 13.07±2.13 ng/mL & 15.53±3.40 ng/mL; 57.49±5.64 pg/mL & 62.61±3.71 pg/mL; 5.12±0.44 mg/dL & 5.35±0.37 mg/dL respectively. The current results suggest that circulating levels of osteoprotegerin play a crucial role in biological mechanism of type (II) diabetes, and are possible biomarkers of insulin resistance and progression of many serious health problems associated with diabetes.

This study aimed at the investigation of abnormal liver and renal functions by biochemical manifestations of underlying metabolic abnormalities in relation to hyperglycemia in non-insulin-dependent diabetic patients. The study comprised 118 diabetic patients (56 males, 62 females) and 60 age-matched healthy non-diabetic controls (30 males, 30 females). All subjects were tested for serum levels of liver enzymatic indicators, which include aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP), as well as non enzymatic parameters, including total bilirubin and total proteins.Also, serum levels of renal function markers, including microalbumin, creatinine, urea, and uric acid were measured. The findings of this study stated that serum ALT, AST, and ALP levels were significantly higher in diabetic males and females with both age ranges (40-59 & 60-80 years). Serum total bilirubin level showed a significant decrease in diabetic males and females of both age groups. However, total proteins level showed a significant increase in diabetic males and females of both age groups. The results also showed that the level of microalbumin in urine as well as those of creatinine, urea, and uric acid in the serum were significantly higher in diabetic males and females. The present study concludes that there is a bidirectional relationship of enzymatic and non enzymatic liver and renal functions markers with the hyperglycemic status in Type 2 diabetes mellitus.

The current study was conducted to investigate the impact of consumption of Moringa oleifera seed on the levels of glucose, Bone function markers and adipokines, in diabetes males. This study involved (47) diabetes male patients aged (40-59) years that have been classified into (Group 1): diabetes male under the treatment of Moringa oleifera seed (n=15) and (Group 2) diabetes male under the treatment of synthetic drugs  (n=32). In addition to (15) apparently healthy subjects as a control group. Diabetes male patients consumed every day the Moringa oleifera seed at dose of 70 mg (one teaspoon) for 6 months. Blood samples were collected from both group to determine serum lipid profile,  adipokines, bone function markers. Results indicate that consumption of Moringa oleifera seed induced a remarkable decrease in the levels of glucose and HbA1c, with accompanying increases in the levels of leptin and adiponectin. Furthermore, the use of Moringa oleifera seed increased vitamin D and inorganic phosphorus levels with concomitant decreases in the levels of osteoprotegerin and serum total calcium. These results show that consumption of Moringa oleifera seeds has a hypoglycemic effect, improving bone function markers and increasing adipokine levels in diabetic patients by improving biochemical indicators.