The current investigation aimed to test the alterations of the levels of obestatin hormone, lipid profile and cardiac function markers in relation to hyperglycemia in patients with non-insulin diabetes mellitus. The study included 118 diabetic subjects (56 males, 62 females) and 60 healthy non-diabetic subjects (30 males, 30 females). Diabetic and healthy subjects were age-matched. Serum levels of obestatin, lipid profile markers including total cholesterol (STC), triglycerides (STG), low and high density lipoproteins (LDL-C and HDL-C), as well as cardiac function markers including, creatine kinase and lactate dehydrogenase enzymes were determined in all subjects. The findings revealed a remarkable decrease in the level of serum obestatin in both diabetic males and females with both age ranges (40-59 and 60-80 years). On the other hand, the results showed that serum STC, LDL-C, and STG levels were statistically significantly elevated, while that for HDL-C was significantly decreased in diabetic males and females with both age ranges. Concerning the cardiac markers, the results found out that the levels of CK-MB and LDH were significantly increased in type 2 diabetic males and females with both age ranges. The results suggested that a low concentration of obestatin is a significant risk factor for type 2 DM, with a key role for this hormone in the pathogenesis. Accordingly, altered levels of obestatin could be used as an important indicator for type 2 DM. The current study also suggests a direct relationship between lipid profiles, except for HDL, the decreased obestatin level, the increased cardiac function markers, and hyperglycemic status in type 2 DM.

Humans always mount a robust immune response to the bacterial infection caused by Helicobacter pylori, which causes various gastrointestinal tract infections. Calprotectin (CALP) and B-Cell Activating Factor (BAFF) are inflammatory biomarkers having a role in the gastrointestinal neutrophilic response to bacterial infection. The study was designed to assess serum CALP and BAFF as inflammatory biomarkers in H. pylori infection and peptic ulcer patients. The current study comprised 112 people, including 62 H. pylori-infected patients (34 men and 28 women) who were clinically diagnosed with H. pylori infection via testing positive for the H. pylori stool antigen test; they were compared to a control group of 50 healthy people (34 men and 16 women) who were age and gender-matched to H. pylori-infected patients. The serum level of CALP and BAFF were assayed using the ELISA technique. The biochemical parameters were statistically compared between patients and controls by unpaired Man-Whitney U t-test and Receiver Operating Characteristic (ROC) curve analysis. There was a significant elevation of serum CALP in H. pylori-infected patients [116.4(120.7), p=0.0132] in comparison to healthy controls [99.50(115.8)]. Similarly, there was a significant elevation of serum BAFF concentration in H. pylori-infected patients [485.7(367.1), p=0.0014] in comparison to healthy controls [444.5(513.0)]. The ROC curve analysis suggests serum CALP and BAFF as reasonable inflammatory biomarkers for H. pylori infection with statistically significant (p=0.0135, p=0.0015) area under the ROC curve of (0.6361, 0.6748), respectively. CALP and BAFF are potent inflammatory biomarkers involved in the development and etiology of H. pylori infection. Serum CALP and BAFF levels could be used as biomarkers for chronic inflammation induced by H. pylori. CALP and BAFF biomarkers can be combined to diagnose and predict the prognosis of H. pylori infection.

Complications associated with diabetes are a consequence of acute disturbance in glucose metabolism in a human body. The most significant complication of diabetes is bone disorders which contributes to high levels of bone disability. This study included 118 diabetic patients, 56 males, 62 females, and 60 healthy non-diabetic controls, 30 males, 30 females. The patients and controls were age matched. Circulating levels of bone function markers (osteoprotegerin, vitamin D, PTH, total calcium and inorganic phosphorus) were determined in all subject groups. The data obtained from this study showed that the serum levels of osteoprotegerin had significantly increased in both diabetic male & female in both age ranges which were 496.3±61.46 pg/mL & 335.7±29.33 pg/mL; 329.8±48.78 pg/mL & 219.9±18.72 pg/mL respectively, in comparison with its level in control matched age ranges 294.6±26.19 pg/mL & 226.8±28.07 pg/mL; 215.7±31.85 pg/mL & 171.9±14.19 pg/mL respectively. Serum calcium concentration had non-significantly increased both in the diabetic males and females in both age ranges which were 11.10±0.46 mg/dL & 11.76±0.74 mg/dL; 10.33±0.33 mg/dL & 10.28±0.48 mg/dL respectively, when compared with its level in control matched age ranges 10.46±0.34 mg/dL & 10.14±0.35 mg/dL;9.69±0.41 mg/dL & 10.08±0.45 mg/dL. Serum vitamin D, parathyroid hormone (PTH) and inorganic phosphorus concentrations had significantly decreased both in the diabetic male and female subjects in both age ranges which were 5.78±1.30 ng/mL and 2.47±0.12 ng/mL; 9.47±1.98 ng/mL, and 10.70±2.11 ng/mL; 74.78±7.42 pg/mL & 67.83±3.69 pg/mL;42.94±2.00 pg/mL & 15.51±1.98 pg/mL; 4.34±0.27 mg/dL & 4.76±0.35 mg/dL; 4.38±0.21 mg/dL; 5.12±0.44 mg/dL respectively when compared with their level in control matched age ranges 13.07±2.13 ng/mL & 15.53±3.40 ng/mL; 57.49±5.64 pg/mL & 62.61±3.71 pg/mL; 5.12±0.44 mg/dL & 5.35±0.37 mg/dL respectively. The current results suggest that circulating levels of osteoprotegerin play a crucial role in biological mechanism of type (II) diabetes, and are possible biomarkers of insulin resistance and progression of many serious health problems associated with diabetes.

This study aimed at the investigation of abnormal liver and renal functions by biochemical manifestations of underlying metabolic abnormalities in relation to hyperglycemia in non-insulin-dependent diabetic patients. The study comprised 118 diabetic patients (56 males, 62 females) and 60 age-matched healthy non-diabetic controls (30 males, 30 females). All subjects were tested for serum levels of liver enzymatic indicators, which include aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP), as well as non enzymatic parameters, including total bilirubin and total proteins.Also, serum levels of renal function markers, including microalbumin, creatinine, urea, and uric acid were measured. The findings of this study stated that serum ALT, AST, and ALP levels were significantly higher in diabetic males and females with both age ranges (40-59 & 60-80 years). Serum total bilirubin level showed a significant decrease in diabetic males and females of both age groups. However, total proteins level showed a significant increase in diabetic males and females of both age groups. The results also showed that the level of microalbumin in urine as well as those of creatinine, urea, and uric acid in the serum were significantly higher in diabetic males and females. The present study concludes that there is a bidirectional relationship of enzymatic and non enzymatic liver and renal functions markers with the hyperglycemic status in Type 2 diabetes mellitus.

The current study was conducted to investigate the impact of consumption of Moringa oleifera seed on the levels of glucose, Bone function markers and adipokines, in diabetes males. This study involved (47) diabetes male patients aged (40-59) years that have been classified into (Group 1): diabetes male under the treatment of Moringa oleifera seed (n=15) and (Group 2) diabetes male under the treatment of synthetic drugs  (n=32). In addition to (15) apparently healthy subjects as a control group. Diabetes male patients consumed every day the Moringa oleifera seed at dose of 70 mg (one teaspoon) for 6 months. Blood samples were collected from both group to determine serum lipid profile,  adipokines, bone function markers. Results indicate that consumption of Moringa oleifera seed induced a remarkable decrease in the levels of glucose and HbA1c, with accompanying increases in the levels of leptin and adiponectin. Furthermore, the use of Moringa oleifera seed increased vitamin D and inorganic phosphorus levels with concomitant decreases in the levels of osteoprotegerin and serum total calcium. These results show that consumption of Moringa oleifera seeds has a hypoglycemic effect, improving bone function markers and increasing adipokine levels in diabetic patients by improving biochemical indicators.

Objectives: This study set out to determine how Calprotectin and B cell activating factor contributes to early COVID-19 patient severity prediction. Methods: The study included 25 healthy controls and 52 patients with SARS-COV2 infection who were clinically diagnosed with COVID-19 illness and were between the ages of 23 and 35. The serum levels of CALP and BAFF were measured using the ELISA method. To gauge CRP levels, an immunoturbidometric assay was performed. Results: Variations in serum levels of CALP and BAFF were found to be statistically insignificant in the study (P=0.7109 & P=0.7575, respectively). When compared to the control group (103.95±36.67 ng/mL; 403.03±1.03), COVID-19 patients had non-significantly raised levels of CALP and BAFF (106.5±4.67 ng/mL; 436.9±12.77 pg/mL, respectively). According to ROC curve analysis, the area under the receiver operating characteristics curve (AUC) for CALP and BAFF was (0.5170) and (0.5259), respectively. (r=0.6923; P=0.0001). There was a significant positive correlation between serum CALP and BAFF levels. The connection between serum CRP levels and CALP (r=0.3010; P=0.1271) and BAFF levels (r=0.2912; P=0.1406) was insignificantly positive. Conclusion: The current study's findings suggested that serum CALP and BAFF concentrations were increased in COVID-19 patients, suggesting that these inflammatory markers may be helpful indicators of the severity of COVID-19.

Eight-hydroxyguanosine  (8-OHdG) is considered as one of the principle forms of oxygen radicals that stimulated the oxidative stress and has been extensively utilized as a biomarker for oncogenesis. The primary goal of the present study was to investigate the alteration in the levels of 8-OHdG, antioxidant profile and proinflammatory cytokines levels in patients with lung carcinoma. Blood samples were collected from 40 cases with lung cancer (stage III) admitted before the treatment, for health examination at the Nanakaly Hospital in Erbil city and 45 healthy samples of controls with ages ranging between 38-69 years for both groups. Circulating concentration of 8-OHdG, tumor necrosis factor and interleukin-6 were evaluated by ELISA. Circulating levels of superoxide dismutase (SOD), peroxidase (POX) and ascorbic acid (vitamin C) levels were also analyzed by using ELISA. The current work proposes that (8-OHdG) can be used as a functional biological marker, considering oxidative stress among the patients with lung carcinoma. The obtained data also indicated a correlation between serum cytokine concentrations and the rate of survival in  lung carcinoma patients.

Objectives: This study set out to determine how Calprotectin and B cell activating factor contributes to early COVID-19 patient severity prediction. Methods: The study included 25 healthy controls and 52 patients with SARS-COV2 infection who were clinically diagnosed with COVID-19 illness and were between the ages of 23 and 35. The serum levels of CALP and BAFF were measured using the ELISA method. To gauge CRP levels, an immunoturbidometric assay was performed. Results: Variations in serum levels of CALP and BAFF were found to be statistically insignificant in the study (P=0.7109 & P=0.7575, respectively). When compared to the control group (103.95±36.67 ng/mL; 403.03±1.03), COVID-19 patients had non-significantly raised levels of CALP and BAFF (106.5±4.67 ng/mL; 436.9±12.77 pg/mL, respectively). According to ROC curve analysis, the area under the receiver operating characteristics curve (AUC) for CALP and BAFF was (0.5170) and (0.5259), respectively. (r=0.6923; P=0.0001). There was a significant positive correlation between serum CALP and BAFF levels. The connection between serum CRP levels and CALP (r=0.3010; P=0.1271) and BAFF levels (r=0.2912; P=0.1406) was insignificantly positive. Conclusion: The current study's findings suggested that serum CALP and BAFF concentrations were increased in COVID-19 patients, suggesting that these inflammatory markers may be helpful indicators of the severity of COVID-19.