Frozen fish exposed to many risks of contamination during long chain of catching, transportation, dressing and freezing from different sources till they reach to consumers which make it harmful or unfit for human consumption.A total of 150 random samples of frozen fish of Mackerel, Saurus, Mugil Cephalus, Horse Mackerel and Sardine (30 of each) were collected from different fish markets at Kaliobia Governorate for determination of Psychrotrophic, Pseudomonas and Aeromonas count in frozen fish samples. The mean value of Psychrotrophic , Pseudomonas and Aeromonas count (cfu/g) were 5.11x10 5 ±2.4x10 5 , 9.