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The aim of this study is to examine the chemical constituents of Heliotropium hirsitutissimum GRAUER naturally spreaded in Denizli and its ecotype spreaded a hotter region in Izmir (Bornova-campus) and to identify them by GC-MS. After the extraction of fresh leaves, one alkaloid and a mixture of phenols, essential oils: 2,3-butanediol, 2-3-dimethyl-(CAS) pinacol (other name: tetramethylethylene glycol), phenol, 4-and 2,6,10,14,18,22-tetracosahexaene (other name: squalene) were identified in the species spreaded naturally in Denizli. Izmir ecotype gave a mixture of essential oils and phenol 1-hexadecanol; tetracosane acid, butyl 2-methylpropyl ester, phenol: phenol, 4,4′-(1methylethylidene)bis-(CAS) 2,2-bis(p-hydroxyphenyl)propane, ethanol, 2-2′-(1-2-ethanediol bis(oxy))bis-(CAS)triethylene glycol, octadecanoic acid (other name stearic acid), 2-hydroxy-1-(hydroxymethyl) ethyl ester (CAS) and 2-Monostearin. The main components of the two ecotypes was 1,2-benzene dicarboxylic acid and hexadecanol. The presence of triethylene glycol was found to be as the significant structural difference of Izmir ecotype. These results indicated that the composition of heliotrops highly dependent on the climate conditions.

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