Background: The human skin and soft tissue infections (SSTIs) caused by microbial pathogens during or after trauma, burn injuries, and surgical procedures result in the production of pus, a white to yellow fluid comprised of dead WBCs, cellular debris, and necrotic tissues.Objective: The purpose of the present study was to see the frequency and distribution of bacteria isolated from pus and sensitivity pattern.Methodology: This retrospective study was conducted in the Department of Microbiology at Shaheed Suhrawardy Medical College, Dhaka from January 2016 to December 2016 for a period of one (01) year. The pus samples were collected from the patients who were visited in outpatient department and were admitted at IPD in Shaheed Suhrawardy Medical College Hospital (ShSMCH), Dhaka with skin and soft tissue infection. Bacteria were detected by culture and biochemical test and antibiotic susceptibility test done by disc diffusion method.Result: A total number of 212 patients presented with wound infection or pus were recruited for this study. Among 212 patients majority were in the age group of 20 to 40 years which was 89(42.0%) cases. Interestingly male was predominant than female which was 119(56.1%) cases and 93(43.9%) cases respectively. aerobic culture was positive in majority cases which were 131(61.8%) cases.Conclusion: In conclusion the most common isolated bacteria after aerobic culture of pus is the Staphylococcus aureus. However the gram positive cocci is less in number than gram negative bacilli.Bangladesh Journal of Infectious Diseases 2018;5(1):10-14

Background: The antibiotic sensitivity pattern of uropathogenic bacteria is varied with different frequency. Objective: The purpose of the study was to determine the pattern of antibiotic sensitivity against uropathogens isolated from a tertiary care hospital of Dhaka City. Methodology: A cross-sectional study was conducted in the Department of Microbiology at Shaheed Suhrawardy Medical College, Dhaka, Bangladesh during period of January 2016 to December 2016. Among 307 consecutive urine samples were collected from patients of UTI in the microbiology laboratory. Samples were mid-stream urine specimens obtained by clean catch method received from various outpatient departments and inpatient wards were transported to the microbiology laboratory in sterile leak proof container were processed immediately. Bacteria were isolated and identified by standard laboratory procedure. Results: Of the 307 urine samples processed 170(55.4%) gave significant growth of pathogens. The prevalence of UTI is high among females (58.2%) than males (41.8%). The commonest isolates were Escherichia coli (71.2%), Pseudomonas (12.4%), Staphylococcus aureus (10.6%), Acinotobactor (4.1%) and Klebsiella (1.8%). Majority (92.4%) of (uropathogens) isolates were found to be MDR. Conclusion: The study revealed that E. coli is the predominant uropathogens of UTIs.

proteomics approach to Unravel the druggable proteins of the emerging pathogen Waddlia chondrophila and drug repositioning on its MurB protein, HELIYON,

Background: Urinary tract infection is very common in both male and female. Objectives: The purpose of the present study was to see the antibiotic sensitivity pattern of isolated from urinary tract infected patients. Methodology: This cross sectional study was carried out in Dhaka Medical College Hospital, Dhaka for a period of 12 months. Clinically diagnosed cases of urinary tract infection irrespective of age and sex having pus cells ≥5/HPF in the deposits of centrifuged urine were selected as study population. Data regarding organisms causing UTI and their antibiotic sensitivity patterns were collected. For urine culture the urine samples were inoculated on HiCrome UTI agar, CLED agar, 5% sheep blood agar and MacConkey's agar media with a calibrated loop having diameter of 1.45 mm which contains 0.001 ml of urine. The inoculation at 37 o C for 24 hours and CFU count of 10

Background: Newly developed KAtex test can be used as a non invasive tool for diagnosis of Kala-azar. Objectives: The aim of the present study was to validate the KAtex method to diagnose VL. Methodology: This was a cross-sectional study carried out in the Department of Microbiology at Dhaka Medical College, Dhaka, Bangladesh in collaboration with the department of Parasitology at Institute of Epidemiology, Disease control and Research (IEDCR), Dhaka, Bangladesh for a period of one year. Clinically suspected Kala-azar (VL) cases of different age and sex attending IEDCR from different Kalaazar endemic areas of Bangladesh were selected for this study. Microscopy and culture was performed with Bone marrow (BM). KAtex was performed with urine sample. Urine samples taken from cases were pretreated to inactivate heat labile materials which might cause a false positive reaction. Antigen which is detected by KAtex is heat stable carbohydrate antigen. Latex sensitized with antibodies raised against Leishmania donovani antigen was mixed with the urine sample on a glass slide. No agglutination indicates absence of antigen in urine. Result: Cases were 130. Among 130 clinically suspected VL cases, 70 (53.85%) cases were BM positive and 60 (46.15%) cases were BM negative. All the 70 BM positive cases were positive by KAtex. Among 60 BM negative cases, 15 were positive by KAtex. The sensitivity of KAtex is 100% and specificity is 75%. Highest percentage (52.86%) of bone marrow positive cases were below 10 years of age group. Conclusion: In conclusion, KAtex test is a good diagnostic tool for the detection of VL.