The present study aimed to elucidate the effect of nutritional alteration on biomass content and azadirachtin production in cell suspensions of the elite neem variety crida-8. Variations in total nitrogen availability in the medium in terms of different ratios of nitrate: ammonium showed that the ratio 4:1 revealed a profound effect, leading to a 1.5-fold increase in the total extracellular azadirachtin production (5.59 mg/l) over the standard MS medium. Reduction in sucrose (15 mg/l) in the medium exhibited a reduction in biomass and absence of azadirachtin, whereas total phosphate reduction raised intracellular azadirachtin production (6.98 mg/l). An altered medium with a nitrate: ammonium ratio of 4:1 coupled with complete elimination of phosphate enhanced biomass by 36% (59.36 g/l).
Fifty one genotypes of chickpea were evaluated for means and components of variability, heritability, genetic advance and interrelationships for various parameters, direct and indirect effect of various parameters for seed yield. Characters like days to maturity, followed by number of pods per plant, number of primary branches, number of secondary branches, number of pods per plant, number of seeds per pod, days to 50% flowering etc.. Grain yield had high positive and significant association with days to 50% flowering, plant height, number of primary branches, number of secondary branches, number of pods per plant, number of seeds per plant, biological yield and harvest index. Path coefficient analysis showed maximum direct contribution towards grain yield per plant with biological yield followed by harvest index.
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