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Objective: To evaluate the acute effect of Kinesio Taping® on peripheral tissue perfusion in women with chronic venous insufficiency (CVI). Methods: This randomized, double–blind controlled trial included 59 women with mild to moderate CVI. They were randomized to a control group (n = 23; 54.08 ± 9.04 years) and Kinesio Taping® group (KT) (n = 36; 55.87 ± 9.97 years). Near–infrared spectroscopy was positioned in the medial gastrocnemius muscle for assessment of resting tissue perfusion 48 h after the first day of evaluation and after placement of the Kinesio Taping® tape. The evaluation also consisted of performing movements of the plethysmography examination. To verify the comparisons of pre and post Kinesio Taping® data, the variation delta was used for analysis. Mann–Whitney U test was performed an an alpha of 5% was considered statistically significant. Results: There wasn not a significant difference between groups regarding the peripheral tissue perfusion evaluation variables Peripheral Oxygen Saturation Difference – DELTA—SPO2: (KT Group 3.21 (0.84 – 3.62); Control 2.21 (1.59 – 4.83), p = 0.219) and Difference in deoxygenate hemoglobin values – DELTA—HHB (units) KT Group −0.62 (−2.14 – 0.67); Control Group −0.07 (−2.15–2.62) p = 0.238). Despite the lack of statistical significance, the KT group had a 785.7% greater drop in HHB values than the control group. Conclusions: Acute use of Kinesio Taping® in women with CVI did not significantly alter tissue perfusion of calf muscles. However, it was possible to observe percentage differences in venous retention to be considered from a clinical point of view. Trial registration: Brazilian Registry of Clinical Trials (REBEC) RBR–9bhbrp.