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Article Number: 803263 Thank you for choosing to publish with us. This is your final opportunity to ensure your article will be accurate at publication. Please review your proof carefully and respond to the queries using the circled tools in the image below, which are available by clicking ''Comment'' from the right-side menu in Adobe Reader DC.* Please use only the tools circled in the image, as edits via other tools/methods can be lost during file conversion. For comments, questions, or formatting requests, please use. Please do not use comment bubbles/sticky notes. Comment *If you do not see these tools, please ensure you have opened this file with Adobe Reader DC, available for free at get.adobe.com/reader or by going to Help > Check for Updates within other versions of Reader. For more detailed instructions, please see us.sagepub.com/ReaderXProofs. No. Query Please confirm that all author information, including names, affiliations, sequence, and contact details, is correct. Please review the entire document for typographical errors, mathematical errors, and any other necessary corrections; check headings, tables, and figures. Please confirm that the Funding and Conflict of Interest statements are accurate. Please ensure that you have obtained and enclosed all necessary permissions for the reproduction of artistic works, (e.g. illustrations, photographs, charts, maps, other visual material, etc.) not owned by yourself. Please refer to your publishing agreement for further information. Please note that this proof represents your final opportunity to review your article prior to publication, so please do send all of your changes now. AQ: 1 Please provide complete reference details for the in-text citation ''Aider and Ounis (2012).''

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The aim of this study was to characterise the bioactive compounds in mate (Ilex paraguariensis St. Hil) extract and in concentrated mate extract obtained by nanofiltration (NF). Also, the impact of NF on the antioxidant activity of both mate extracts was evaluated in vitro and using eukaryotic cells of Saccharomyces cerevisiae (yeast assay). The results showed a significant increase in the contents of total phenolics (338%), chlorogenic acid (483%), theobromine (323%), caffeine (251%), chlorophyll (321%), condensed tannins (278%) and saponins (211%) in the concentrated mate extract. The concentrated mate extract showed higher in vitro antioxidant activity than the mate extract. According to the results obtained, it can be stated that the use of nanofiltration membrane is a valid approach for the concentration of biologically active compounds in aqueous extract of mate.

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